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产品描述：人肺表面活性物质相关蛋白A(SP-A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液样本中的SFTPA1含量。人肺表面活性物质相关蛋白A（SP-A）是肺泡表面活性物质的主要蛋白成分，具有降低肺泡表面张力、调节表面活性磷脂合成与分泌等作用。研究显示，SP-A在肺损伤和慢性阻塞性肺疾病中发挥重要作用，其基因多态性与疾病易感性相关。试剂盒检测范围为15.6 pg/mL-1000pg/mL，该产品适用于基础科研中探索SP - A在呼吸系统疾病（如肺炎、急性呼吸窘迫综合征、肺纤维化）中的生物学功能，或用于药物开发、毒理学研究中评估肺组织损伤及修复的生物标志物变化。其广泛的样本兼容性为细胞模型、动物实验及临床前研究提供灵活选择，尤其适合需要高灵敏度定量分析的实验场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SFTPA1; COLEC4; PSAP; SFTP1; SFTPA; SFTPA1B; Pulmonary surfactant-associated protein A1; PSP-A; PSPA; SP-A; SP-A1; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; Collectin-4
缩写：SFTPA1
Uniprot No.：Q8IWL2
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine
检测范围：15.6 pg/mL-1000pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:100 | Average % | 86 | |
| Range % | 80-92 | ||
| 1:200 | Average % | 97 | |
| Range % | 91-105 | ||
| 1:400 | Average % | 99 | |
| Range % | 92-110 | ||
| 1:800 | Average % | 93 | |
| Range % | 86-98 |
回收率：
| The recovery of human SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.4202.4182.4192.318 5002.0081.9992.0041.903 2501.5821.5551.5691.468 1251.2481.2111.2301.129 62.50.7090.6890.6990.598 31.250.4720.4360.4540.353 15.60.2900.2690.2800.179 00.1040.0980.101 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human SP-A antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled SP-A antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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