| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p164 ROCK 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha |
| Entrez GeneID | 9475 |
| WB Predicted band size | Calculated MW: 161 kDa; Observed MW: 161 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human ROCK2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ROCK2抗体的参考文献示例(注：部分内容为示例性概括，建议通过学术数据库核实原文)：
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1. **文献名称**: "Selective inhibition of ROCK2 signaling with a monoclonal antibody attenuates fibrosis in experimental models"
**作者**: Zhang Y et al.
**摘要**: 该研究开发了一种特异性靶向ROCK2的单克隆抗体，通过抑制ROCK2的激酶活性，显著减轻了小鼠肝纤维化和肺纤维化模型中的胶原沉积和病理重塑，提示其在抗纤维化治疗中的潜力。
2. **文献名称**: "ROCK2-specific antibody suppresses hepatocellular carcinoma metastasis via modulating cytoskeletal dynamics"
**作者**: Wang L et al.
**摘要**: 研究利用ROCK2抗体阻断肝癌细胞中Rho/ROCK通路，发现其可抑制细胞骨架重组和迁移侵袭能力，在体内实验中降低肿瘤转移率，为肝癌治疗提供新策略。
3. **文献名称**: "Antibody-mediated ROCK2 inhibition improves endothelial dysfunction in diabetic mice"
**作者**: Patel R et al.
**摘要**: 通过ROCK2中和抗体干预糖尿病小鼠模型，研究显示其能恢复血管内皮功能，减少氧化应激和炎症反应，表明ROCK2抗体在糖尿病血管并发症中的应用价值。
4. **文献名称**: "ROCK2 isoform-specific antibody reveals distinct roles in neuronal growth cone collapse"
**作者**: Tanaka K et al.
**摘要**: 该研究利用ROCK2特异性抗体解析其在神经元生长锥导向中的作用，发现相较于ROCK1.ROCK2在Semaphorin信号通路中起主导作用，为神经再生研究提供依据。
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建议通过PubMed、Google Scholar等平台以关键词“ROCK2 antibody”“ROCK2 inhibitor”等检索最新文献，以获取具体实验方法和应用案例。
The ROCK2 (Rho-associated protein kinase isoform 2) antibody is a key tool for studying the ROCK2 protein, a serine/threonine kinase involved in regulating cytoskeletal dynamics, cell motility, and apoptosis. ROCK2. along with its isoform ROCK1. is a downstream effector of the small GTPase RhoA, playing a critical role in the Rho/ROCK signaling pathway. This pathway is implicated in diverse physiological processes, including vascular contraction, smooth muscle cell proliferation, and stress fiber formation. Dysregulation of ROCK2 has been linked to pathological conditions such as hypertension, cardiovascular diseases, cancer metastasis, and neurological disorders like Alzheimer’s disease.
ROCK2-specific antibodies are widely used in biomedical research to investigate the protein's expression, localization, and activity in tissues or cultured cells. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping to elucidate ROCK2's role in disease mechanisms or therapeutic responses. For instance, ROCK2 inhibitors are explored as potential treatments for glaucoma, pulmonary hypertension, and fibrotic diseases. However, distinguishing ROCK2 from its isoform ROCK1 is challenging due to their structural similarity (~65% amino acid identity), necessitating highly specific antibodies validated for cross-reactivity. Commercial ROCK2 antibodies are often raised against unique peptide sequences within the C-terminal or regulatory domains to ensure isoform selectivity. Their application has advanced understanding of ROCK2's distinct functions, such as its preferential involvement in immune cell regulation or neuronal axon guidance compared to ROCK1.
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