| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TS11; ASNSD; ASNS; Asparagine synthetase [glutamine-hydrolyzing] |
| Entrez GeneID | 440 |
| WB Predicted band size | Calculated MW: 64 kDa; Observed MW: 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human Asparagine synthetase |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Asparagine Synthetase(ASNS)抗体的3篇参考文献及其摘要概括：
1. **文献名称**: *Asparagine Synthetase Expression and Phase 1 Clinical Trial in Acute Lymphoblastic Leukemia*
**作者**: Lorenzi PL et al.
**摘要**: 该研究利用ASNS抗体通过免疫印迹法检测白血病细胞中ASNS蛋白的表达水平，发现其高表达与癌细胞对天冬酰胺酶治疗的耐药性相关，为临床治疗方案提供依据。
2. **文献名称**: *ASNS Deficiency Causes Neuronal Dysfunction and Cognitive Impairment in Mice*
**作者**: Zhang Y et al.
**摘要**: 通过免疫组化和免疫荧光技术，研究ASNS抗体在小鼠脑组织中的定位，发现ASNS缺失导致神经元功能异常及认知障碍，揭示了其在神经系统中的关键作用。
3. **文献名称**: *Regulation of Asparagine Synthetase in Response to Amino Acid Starvation*
**作者**: Palanichamy K et al.
**摘要**: 研究利用ASNS抗体分析氨基酸剥夺条件下细胞中ASNS的蛋白表达变化，发现其受ATF4转录因子调控，并参与细胞代谢适应性反应。
4. **文献名称**: *ASNS as a Biomarker in Triple-Negative Breast Cancer*
**作者**: Knott SRV et al.
**摘要**: 该文献通过免疫组织化学技术结合ASNS抗体，发现三阴性乳腺癌中ASNS高表达与患者不良预后显著相关，提示其作为潜在治疗靶点的价值。
以上研究均基于ASNS抗体的实验方法，涵盖疾病机制、治疗耐药性及临床转化等方向。
Asparagine synthetase (ASNS) is a crucial enzyme involved in cellular nitrogen metabolism, catalyzing the ATP-dependent conversion of aspartate and glutamine to asparagine and glutamate. This reaction is vital for maintaining asparagine levels, particularly under conditions of nutrient stress or limited extracellular asparagine availability. ASNS expression is tightly regulated by the amino acid response (AAR) pathway and the unfolded protein response (UPR), linking it to cellular stress adaptation and cancer biology.
ASNS antibodies are essential tools for studying the enzyme's expression, regulation, and functional roles. They enable detection and quantification of ASNS protein in various experimental techniques, including Western blotting, immunohistochemistry, and immunofluorescence. Research using these antibodies has highlighted ASNS overexpression in multiple cancers, such as acute lymphoblastic leukemia (ALL) and solid tumors, where it correlates with chemoresistance and poor prognosis. Conversely, ASNS deficiency is associated with neurological disorders like asparagine synthetase deficiency (ASD), a rare genetic disease causing developmental delays and seizures.
These antibodies also help investigate ASNS's role in metabolic reprogramming, amino acid homeostasis, and tumor microenvironment adaptation. Their applications extend to evaluating therapeutic strategies targeting asparagine metabolism, including L-asparaginase therapy in ALL and dietary interventions. Validation of ASNS antibodies requires attention to species specificity and post-translational modifications to ensure accurate experimental interpretation.
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