| WB | 咨询技术 | Rat |
| IF | 1/20 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | ARP; Arp1; ARL18 |
| Entrez GeneID | 10139 |
| WB Predicted band size | Calculated MW: 23 kDa; Observed MW: 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | A synthetic peptide of human ARFRP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ARFRP1抗体的模拟参考文献示例(注：文献为虚构，仅供示例参考)：
1. **文献名称**：*ARFRP1 regulates insulin secretion through vesicular trafficking in pancreatic β-cells*
**作者**：Müller T, et al.
**摘要**：本研究利用ARFRP1特异性抗体(兔多克隆，Sigma-Aldrich)进行Western blot和免疫荧光实验，发现ARFRP1通过调控胰岛素囊泡的高尔基体运输影响分泌功能，敲除ARFRP1导致小鼠β细胞分泌缺陷。
2. **文献名称**：*ARFRP1 modulates adipocyte lipid metabolism via interaction with COP-I vesicles*
**作者**：Kim S, et al.
**摘要**：通过ARFRP1抗体(小鼠单克隆，Abcam)的免疫共沉淀和免疫组化实验，揭示ARFRP1在脂肪细胞中与COP-I复合物互作，调控脂滴形成及脂肪酸代谢，其表达异常与肥胖相关。
3. **文献名称**：*ARFRP1 promotes hepatocellular carcinoma metastasis by enhancing EGFR signaling*
**作者**：Chen L, et al.
**摘要**：使用ARFRP1抗体(货号#sc-100. Santa Cruz)进行组织芯片分析，发现ARFRP1在肝癌中高表达，并通过增强EGFR膜定位促进肿瘤侵袭，提示其作为预后标志物的潜力。
4. **文献名称**：*ARFRP1 knockout mice exhibit disrupted Golgi structure and neuronal defects*
**作者**：Yamamoto K, et al.
**摘要**：通过ARFRP1抗体验证敲除小鼠模型，结合电镜和免疫荧光，证明ARFRP1缺失导致神经元高尔基体碎片化，引发轴突运输障碍及运动功能异常。
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注：以上文献信息为示例性虚构内容，实际研究中请通过学术数据库检索具体文献。
The ARFRP1 (ADP-ribosylation factor-related protein 1) antibody is a tool used to detect and study the ARFRP1 protein, a member of the ADP-ribosylation factor (ARF) family involved in intracellular vesicular trafficking and membrane dynamics. ARFRP1 plays a regulatory role in Golgi-to-ER retrograde transport and interacts with ARF-GTPases to modulate secretory pathways. Research has linked ARFRP1 to metabolic regulation, particularly in glucose homeostasis, as knockout mouse models exhibit severe hypoglycemia and disrupted insulin secretion.
This antibody is essential for investigating ARFRP1's expression, localization, and function in cellular and disease contexts. Studies using ARFRP1 antibodies have revealed its overexpression in certain cancers, including gastric and colorectal cancers, suggesting potential roles in tumor progression. It is also implicated in lipid metabolism through its interaction with ARF-like proteins and effects on lipolytic pathways.
Available as polyclonal or monoclonal variants, ARFRP1 antibodies are validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. Their development has advanced understanding of ARFRP1's molecular mechanisms, including its involvement in the Akt/PKB signaling pathway and vesicle coat assembly. Researchers rely on these antibodies to explore ARFRP1's clinical relevance, particularly in metabolic disorders and oncogenesis, highlighting its dual role as a cellular trafficking regulator and disease biomarker.
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