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产品描述：人分泌型卷曲相关蛋白5(SFRP5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SFRP5含量。SFRP5是一种分泌型糖蛋白，属于分泌型卷曲相关蛋白家族。它可通过拮抗Wnt信号通路发挥作用。在研究中发现，其与代谢、炎症等多种生理病理过程相关，有望成为治疗肥胖、糖尿病、心血管疾病等的潜在靶点。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于体外实验环境下多种生物样本的 SFRP5 表达水平检测，可为代谢综合征、动脉粥样硬化等疾病的分子机制研究及药物开发提供数据支持；支持科研人员高效开展细胞模型、动物模型或临床前样本的 SFRP5 动态变化分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SFRP5 ELISA Kit; FRP1B ELISA Kit; SARP3 ELISA Kit; Secreted frizzled-related protein 5 ELISA Kit; sFRP-5 ELISA Kit; Frizzled-related protein 1b ELISA Kit; FRP-1b ELISA Kit; Secreted apoptosis-related protein 3 ELISA Kit; SARP-3 ELISA Kit
缩写：SFRP5
Uniprot No.：Q5T4F7
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SFRP5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 81-95 | |||||
| 1:400 | Average % | 96 | ||||
| Range % | 90-105 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 90-110 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human SFRP5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-98 | ||||
| EDTA plasma (n=4) | 91 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.4912.3522.4222.241502.2322.1762.2042.023251.8521.7841.8181.63712.51.0611.0391.0500.8696.250.6280.5940.6110.4303.120.3740.3590.3670.1861.560.2460.2290.2380.05700.1850.1770.181 | ||||||
货期：3-5 working days
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