首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人分泌型卷曲相关蛋白2(SFRP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的SFRP2含量。SFRP2是一种分泌型糖蛋白，属于分泌型卷曲相关蛋白家族。它参与Wnt信号通路的调控，在胚胎发育等过程中发挥重要作用。研究发现其异常表达与肿瘤等多种疾病的发生发展相关，深入研究其作用机制有望为疾病治疗提供新靶点与策略。试剂盒检测范围为0.156 ng/mL-10 ng/mL，在科研领域可应用于肿瘤微环境分析、干细胞分化研究、骨骼发育调控等方向，尤其适合探究SFRP2在结直肠癌、骨质疏松等疾病模型中的表达变化及其功能机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AI851596 ELISA Kit; Frizzled related protein 2 ELISA Kit; FRP 2 ELISA Kit; FRP-2 ELISA Kit; FRP2 ELISA Kit; MGC128911 ELISA Kit; MGC153618 ELISA Kit; MGC53344 ELISA Kit; SARP 1 ELISA Kit; SARP-1 ELISA Kit; SARP1 ELISA Kit; SDF 5 ELISA Kit; SDF5 ELISA Kit; Secreted apoptosis related protein 1 ELISA Kit; Secreted apoptosis-related protein 1 ELISA Kit; Secreted frizzled related protein 2 ELISA Kit; Secreted frizzled-related protein 2 ELISA Kit; sFRP 2 ELISA Kit; sFRP-2 ELISA Kit; SFRP2 ELISA Kit; Sfrp2 protein ELISA Kit; SFRP2_HUMAN ELISA Kit
缩写：SFRP2
Uniprot No.：Q96HF1
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SFRP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 85-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 96-104 | |||||
回收率：
| The recovery of human SFRP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.2062.1192.1632.02651.5351.6501.5931.4562.51.1251.1031.1140.9771.250.7660.7450.7560.6190.6250.5210.5540.5380.4010.3120.3500.3270.3390.2020.1560.2530.2680.2610.12400.1380.1360.137 | ||||||
货期：3-5 working days
×
