首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人分泌型卷曲相关蛋白4(SFRP4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFRP4含量。SFRP4是一个重要靶点。其它属于分泌型卷曲相关蛋白家族，在多种生理和病理过程中发挥作用。研究机制上，主要通过与Wnt信号通路相互作用来调节细胞的增殖、分化等行为，对其深入研究有助于疾病治疗策略的开发。试剂盒检测范围为1.56 ng/mL-100ng/mL，适用于癌症微环境研究、代谢调控机制探索或骨发育相关模型中SFRP4蛋白表达水平的动态分析，为靶点功能研究及疾病生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Frizzled protein ELISA Kit; Frizzled protein human endometrium ELISA Kit; FRP 4 ELISA Kit; FRP4 ELISA Kit; FrpHE ELISA Kit; human endometrium ELISA Kit; PYL ELISA Kit; Secreted frizzled-related protein 4 ELISA Kit; sFRP-4 ELISA Kit; Sfrp4 ELISA Kit; SFRP4_HUMAN ELISA Kit
缩写：SFRP4
Uniprot No.：Q6FHJ7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SFRP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:10 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:40 | Average % | 88 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of human SFRP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 83-95 | ||||
| EDTA plasma (n=4) | 99 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.7122.6522.6822.570501.8851.7761.8311.719251.2121.2041.2081.09612.50.6210.6640.6430.5316.250.3830.3570.3700.2583.120.2970.2780.2880.1761.560.2090.2120.2110.09900.1160.1070.112 | ||||||
货期：3-5 working days
×
