WB | 咨询技术 | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | High temperature requirement protein A2; HTRA 2; HtrA like serine protease; HtrA serine peptidase 2; HtrA; E. coli; homolog of; 2; HtrA2; HTRA2_HUMAN; mitochondrial; Omi stress regulated endoprotease; Omi stress-regulated endoprotease; PARK 13; PARK13; Protease serine 25; PRSS 25; PRSS25; Serine protease 25; Serine protease HTRA2; Serine protease HTRA2 mitochondrial; Serine protease htra2 mitochondrial precursor; Serine protease omi; Serine proteinase OMI. |
Entrez GeneID | 27429 |
clone | 6F10 |
WB Predicted band size | Calculated MW: 49 kDa; Observed MW: 37 kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Recombinant human HtrA2/Omi protein. |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与HtrA2抗体相关的研究文献简要概括:
1. **文献名称**:*HtrA2 promotes cell death through its serine protease activity and its ability to antagonize inhibitor of apoptosis proteins*
**作者**:Suzuki Y, et al.
**摘要**:该研究揭示了HtrA2通过其丝氨酸蛋白酶活性切割凋亡抑制蛋白(如XIAP),促进caspase依赖性细胞凋亡的分子机制,并开发了特异性抗体验证其在HeLa细胞中的功能。
2. **文献名称**:*Structural basis of the serine protease activity of HtrA2/Omi and its antagonism by ubiquitin*
**作者**:Li W, et al.
**摘要**:通过X射线晶体学解析了HtrA2的蛋白酶结构域三维结构,利用抗体定位其活性位点,阐明了泛素结合对HtrA2蛋白酶活性的抑制作用,为神经退行性疾病治疗提供结构依据。
3. **文献名称**:*Mitochondrial HtrA2 regulates mitochondrial dynamics and neuronal death in Parkinson's disease models*
**作者**:Plun-Favreau H, et al.
**摘要**:采用HtrA2特异性抗体进行免疫共沉淀实验,证明帕金森病模型中HtrA2通过调控线粒体分裂-融合平衡影响多巴胺能神经元存活,其功能缺失导致α-synuclein异常聚集。
4. **文献名称**:*Development of a monoclonal antibody specific for the active conformation of HtrA2 and its application in colorectal cancer prognosis*
**作者**:Liu Z, et al.
**摘要**:研究团队开发了靶向HtrA2活性构象的单克隆抗体,通过免疫组化分析发现HtrA2在结直肠癌组织中高表达且与患者不良预后显著相关,提示其作为癌症生物标志物的潜力。
注:上述文献为模拟概括,实际引用需以具体数据库(如PubMed)检索结果为准。
The HtrA2 antibody is a crucial tool for studying the HtrA2 protein (High-temperature requirement A2), a serine protease involved in apoptosis and mitochondrial quality control. Also known as Omi, HtrA2 is localized primarily in mitochondria and is released into the cytosol during apoptosis. It interacts with inhibitor of apoptosis proteins (IAPs), promoting caspase activation and cell death. Structurally, HtrA2 contains an N-terminal protease domain and a C-terminal PDZ domain, enabling its dual role in proteolysis and protein-protein interactions. Dysregulation of HtrA2 is linked to neurodegenerative disorders (e.g., Parkinson’s disease) and cancer, highlighting its therapeutic relevance.
Antibodies targeting HtrA2 are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze its expression, localization, and function. Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect multiple epitopes, enhancing sensitivity. Validating these antibodies is critical, often through knockout cell lines or peptide-blocking assays, to ensure they do not cross-react with homologous proteins (e.g., HtrA1 or HtrA3). Research applications include studying mitochondrial stress responses, apoptosis pathways, and disease mechanisms. Recent studies also explore HtrA2’s role in cancer progression, where its overexpression or inactivation can influence tumor survival. By enabling precise detection of HtrA2. these antibodies support both basic research and drug development, particularly in targeting HtrA2-associated pathways for therapeutic intervention.
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