首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠可溶性血纤蛋白单体复合物(SFMC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFMC含量。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，适用于血栓形成机制研究、心血管疾病动物模型评估、抗凝血药物筛选等科研领域，特别在深静脉血栓、弥散性血管内凝血（DIC）等病理模型的分子机制研究中具有重要应用价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：SFMC
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 ng/mL-4000 ng/mL
灵敏度：15.6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SFMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:200 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:400 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of rat SFMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 40002.6552.5982.6272.433 20002.3622.3562.3592.165 10001.9071.9631.9351.741 5001.1531.1781.1660.972 2500.8350.8320.8340.640 1250.4620.4980.4800.286 62.50.3340.3390.3370.143 00.1930.1950.194 | ||||||
货期：3-5 working days
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