| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1 |
| Entrez GeneID | 1642 |
| clone | 2G6 |
| WB Predicted band size | Calculated MW: 127 kDa; Observed MW: 127 kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant human DDB1 protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于DDB1抗体的3篇代表性文献的简要总结：
1. **文献名称**: "CUL4A ubiquitin ligase is recruited to DDB1 through adaptor protein DDB2 to regulate replication licensing"
**作者**: Jin J, Arias EE, Chen J, et al.
**摘要**: 该研究利用DDB1抗体通过免疫沉淀技术，发现DDB1-CUL4A复合物通过DDB2适配蛋白结合到染色质，调控DNA复制起始点的许可机制，揭示了其在细胞周期中的关键作用。
2. **文献名称**: "DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases"
**作者**: He YJ, McCall CM, Hu J, et al.
**摘要**: 通过DDB1抗体的免疫共沉淀实验，作者证明DDB1作为支架蛋白，将多种含WD40结构域的底物受体(如CSA、DDB2)连接到CUL4泛素连接酶复合物，参与DNA修复和表观遗传调控。
3. **文献名称**: "Human immunodeficiency virus type 1 Vpr interacts with HHR23A, a cellular protein implicated in nucleotide excision DNA repair"
**作者**: Withers-Ward ES, Jowett JB, Stewart SA, et al.
**摘要**: 该研究使用DDB1抗体进行蛋白质相互作用分析，发现HIV-1病毒蛋白Vpr通过直接结合DDB1-HHR23A复合物，干扰宿主细胞的DNA损伤修复通路，促进病毒免疫逃逸。
注：以上为简化摘要，实际文献内容需结合实验细节。如需全文或更多文献，建议通过PubMed/Google Scholar检索关键词"DDB1 antibody"或"DDB1 function"。
The DDB1 (Damage-specific DNA-binding protein 1) antibody is a tool used to study the DDB1 protein, a key component of the CUL4-DDB1 ubiquitin ligase complex. DDB1 was initially identified for its role in nucleotide excision repair (NER), where it forms a heterodimer with DDB2 to recognize UV-induced DNA lesions, such as cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts. Beyond DNA repair, DDB1 acts as an adaptor protein that bridges cullin 4 (CUL4) to substrate receptors (e.g., DCAFs, DDB2), facilitating the ubiquitination and degradation of target proteins involved in diverse cellular processes, including cell cycle regulation, chromatin remodeling, and antiviral responses.
DDB1 antibodies are widely employed in research to investigate its expression, localization, and interactions in contexts like cancer, viral infection, and developmental biology. Dysregulation of DDB1 is linked to genomic instability, impaired DNA damage response, and diseases such as xeroderma pigmentosum (XP) and certain cancers. These antibodies are critical for techniques like Western blotting, immunofluorescence, co-immunoprecipitation, and chromatin immunoprecipitation (ChIP), aiding in the exploration of its roles in proteostasis, transcriptional regulation, and host-pathogen interactions. Studies also highlight DDB1's involvement in hijacking by viral proteins to counteract host defenses, underscoring its therapeutic relevance.
×
