首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠臂板蛋白3A(SEMA3A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SEMA3A含量。大鼠臂板蛋白3A（SEMA3A）是一种分泌型糖蛋白，参与神经元引导和发育，与多种病理生理过程相关，如糖尿病肾病和神经病变。研究显示，SEMA3A通过调节细胞增殖、迁移和凋亡，影响视网膜神经节细胞凋亡和炎症反应，可能成为糖尿病视网膜病变的治疗靶点。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于基础研究中对神经退行性疾病模型、心血管病理机制或肿瘤微环境中SEMA3A动态变化的定量分析，亦可用于药物干预或基因修饰实验中蛋白表达调控的效果评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sema3a ELISA Kit; Semaphorin-3A ELISA Kit; Semaphorin III ELISA Kit; Sema III ELISA Kit
缩写：SEMA3A
Uniprot No.：Q63548
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SEMA3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 87-96 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 83-92 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of rat SEMA3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 80-91 | |||||
| EDTA plasma (n=4) | 92 | 87-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected5002.3242.2542.2892.1562501.6771.5551.6161.4831251.0190.9790.9990.86662.50.5820.5700.5760.44331.20.3670.3630.3650.23215.60.2430.2390.2410.1087.80.1940.1880.1910.05800.1340.1320.133 | |||||||
货期：3-5 working days
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