首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肾素(Renin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的REN含量。试剂盒检测范围为15.6 mIU/mL-1000 mIU/mL，该产品广泛应用于科研领域，如高血压发病机制研究、心血管疾病模型建立、肾脏功能评估，以及药物开发中肾素相关靶点的疗效验证，兼容多种生物样本类型的特点使其能够满足体外实验、动物模型及组织水平的研究需求。试剂盒提供标准品和检测所需全部组分，操作流程便捷，适用于生命科学基础研究与生物医学应用探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Angiotensin forming enzyme ELISA Kit; Angiotensin forming enzyme precursor ELISA Kit; Angiotensinogenase ELISA Kit; Angiotensinogenase precursor ELISA Kit; FLJ10761 ELISA Kit; HNFJ2 ELISA Kit; REN ELISA Kit; Ren1 ELISA Kit; RENI_HUMAN ELISA Kit; Renin ELISA Kit; Renin precursor renal ELISA Kit
缩写：REN
Uniprot No.：P00797
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 mIU/mL-1000 mIU/mL
灵敏度：3.9 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human REN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-100 | |||||
回收率：
| The recovery of human REN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected10002.9532.7652.8592.7135002.5652.4122.4892.3432501.8861.7961.8411.6951250.9750.9430.9590.81362.50.5010.4820.4920.34631.20.3090.3010.3050.15915.60.2220.2180.2200.07400.1470.1450.146 | ||||||
货期：3-5 working days
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