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Human nuclear factor-κB p65,NF-κB p65 ELISA Kit

  • 中文名: 人核因子-κB亚基p65亲和肽(NF-κB p65)ELISA Kit
货号: IPD-E08787h
Price: ¥2500
数量:
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产品详情

  • 产品描述:人核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的RELA含量。RELA是重要靶点。它属于NF-κB家族成员,在细胞的增殖、分化、凋亡及炎症反应等过程中起关键作用。研究机制主要围绕其信号通路展开,聚焦于激活调控、与其他蛋白相互作用等方面,以探索相关疾病治疗策略。试剂盒检测范围为47 pg/mL-3000 pg/mL,支持科研人员探究NF - κB信号通路在疾病模型或药物干预中的调控作用;适用于基础研究领域,如炎症相关疾病机制解析、免疫细胞功能研究或药物筛选中的生物标志物检测,为体外实验提供稳定可靠的蛋白定量解决方案。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:Avian reticuloendotheliosis viral (v rel) oncogene homolog A ELISA Kit; MGC131774 ELISA Kit; NF kappa B p65delta3 ELISA Kit; nfkappabp65 ELISA Kit; NFkB p65 ELISA Kit; NFKB3 ELISA Kit; Nuclear factor kappaB ELISA Kit; Nuclear Factor NF Kappa B p65 Subunit ELISA Kit; Nuclear factor NF-kappa-B p65 subunit ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 ELISA Kit; OTTHUMP00000233473 ELISA Kit; OTTHUMP00000233474 ELISA Kit; OTTHUMP00000233475 ELISA Kit; OTTHUMP00000233476 ELISA Kit; OTTHUMP00000233900 ELISA Kit; p65 ELISA Kit; p65 NF kappaB ELISA Kit; p65 NFkB ELISA Kit; relA ELISA Kit; TF65_HUMAN ELISA Kit; Transcription factor NFKB3 ELISA Kit; Transcription factor p65 ELISA Kit; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) ELISA Kit; V rel avian reticuloendotheliosis viral oncogene homolog A ELISA Kit; v rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit; V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 ELISA Kit

  • 缩写:RELA

  • Uniprot No.:Q04206

  • 种属:Homo sapiens (Human)

  • 样本类型:serum, plasma, tissue homogenates

  • 检测范围:47 pg/mL-3000 pg/mL

  • 灵敏度:11.75 pg/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Immunology

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)
    1:1Average %88
    Range %83-92
    1:2Average %101
    Range %95-104
    1:4Average %102
    Range %98-105
    1:8Average %95
    Range %90-98
  • 回收率:

    The recovery of human NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9389-96
    EDTA plasma (n=4)9591-98














  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected30002.7512.7322.7422.61815001.9661.8761.9211.7977501.3561.3441.3501.2263750.7900.7770.7840.660187.50.4210.4030.4120.288940.3230.3120.3180.194470.2090.1960.2030.07900.1250.1230.124 






  • 货期:3-5 working days

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