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产品描述:人核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的RELA含量。RELA是重要靶点。它属于NF-κB家族成员,在细胞的增殖、分化、凋亡及炎症反应等过程中起关键作用。研究机制主要围绕其信号通路展开,聚焦于激活调控、与其他蛋白相互作用等方面,以探索相关疾病治疗策略。试剂盒检测范围为47 pg/mL-3000 pg/mL,支持科研人员探究NF - κB信号通路在疾病模型或药物干预中的调控作用;适用于基础研究领域,如炎症相关疾病机制解析、免疫细胞功能研究或药物筛选中的生物标志物检测,为体外实验提供稳定可靠的蛋白定量解决方案。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Avian reticuloendotheliosis viral (v rel) oncogene homolog A ELISA Kit; MGC131774 ELISA Kit; NF kappa B p65delta3 ELISA Kit; nfkappabp65 ELISA Kit; NFkB p65 ELISA Kit; NFKB3 ELISA Kit; Nuclear factor kappaB ELISA Kit; Nuclear Factor NF Kappa B p65 Subunit ELISA Kit; Nuclear factor NF-kappa-B p65 subunit ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 ELISA Kit; OTTHUMP00000233473 ELISA Kit; OTTHUMP00000233474 ELISA Kit; OTTHUMP00000233475 ELISA Kit; OTTHUMP00000233476 ELISA Kit; OTTHUMP00000233900 ELISA Kit; p65 ELISA Kit; p65 NF kappaB ELISA Kit; p65 NFkB ELISA Kit; relA ELISA Kit; TF65_HUMAN ELISA Kit; Transcription factor NFKB3 ELISA Kit; Transcription factor p65 ELISA Kit; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) ELISA Kit; V rel avian reticuloendotheliosis viral oncogene homolog A ELISA Kit; v rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit; V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 ELISA Kit
缩写:RELA
Uniprot No.:Q04206
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:47 pg/mL-3000 pg/mL
灵敏度:11.75 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 88 | ||||
Range % | 83-92 | |||||
1:2 | Average % | 101 | ||||
Range % | 95-104 | |||||
1:4 | Average % | 102 | ||||
Range % | 98-105 | |||||
1:8 | Average % | 95 | ||||
Range % | 90-98 |
回收率:
The recovery of human NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 89-96 | ||||
EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2AverageCorrected30002.7512.7322.7422.61815001.9661.8761.9211.7977501.3561.3441.3501.2263750.7900.7770.7840.660187.50.4210.4030.4120.288940.3230.3120.3180.194470.2090.1960.2030.07900.1250.1230.124 |
货期:3-5 working days
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