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产品描述：人再生基因蛋白IV(REG-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的REG4含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于探究 REG - 4 在胃肠道疾病模型中的表达调控机制、肿瘤微环境中的生物学功能，或作为炎症性肠病、肿瘤标志物研究的辅助工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gastrointestinal secretory protein ELISA Kit; GISP ELISA Kit; Reg IV ELISA Kit; REG like protein ELISA Kit; REG-4 ELISA Kit; REG-like protein ELISA Kit; Reg4 ELISA Kit; REG4_HUMAN ELISA Kit; Regenerating gene type IV ELISA Kit; Regenerating islet derived family member 4 ELISA Kit; Regenerating islet derived protein 4 precursor ELISA Kit; Regenerating islet-derived protein 4 ELISA Kit; Regenerating islet-derived protein IV ELISA Kit; REGIV ELISA Kit; RELP ELISA Kit
缩写：REG4
Uniprot No.：Q9BYZ8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human REG-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-92 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 90-95 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of human REG-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 89-94 | ||||
| EDTA plasma (n=4) | 92 | 90-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.8322.8012.8172.676102.2382.1872.2132.07251.4191.3851.4021.2612.50.6730.6540.6640.5231.250.4340.4110.4230.2820.6250.2810.2690.2750.1340.3120.2110.2020.2070.06600.1430.1390.141 | ||||||
货期：3-5 working days
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