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Human Reelin(RELN) ELISA kit

  • 中文名: 人络丝蛋白(RELN)酶联免疫试剂盒
货号: IPD-EL019557HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人络丝蛋白(RELN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的RELN含量。RELN(Reelin)是一种细胞外基质丝氨酸蛋白酶,对大脑皮层和小脑神经元的分层组织发挥关键作用,影响微管功能和神经元迁移。研究显示,RELN在可卡因成瘾机制中扮演关键角色,可能成为潜在治疗靶点。试剂盒检测范围为0.009 ng/ml- 7 ng/ml,适用于神经生物学领域的基础研究,如神经发育机制解析、神经退行性疾病模型建立、精神类疾病分子标志物筛选等方向,也可用于肿瘤微环境研究或心血管疾病模型中RELN蛋白动态变化的定量分析。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    LIS2 ELISA Kit; PRO1598 ELISA Kit; Reeler ELISA Kit; Reelin ELISA Kit; RELN ELISA Kit; RELN_HUMAN ELISA Kit; RL ELISA Kit
  • 缩写:
    RELN
  • Uniprot No.:
    P78509
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.009 ng/ml - 7 ng/ml
  • 灵敏度:
    0.009 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human RELN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    89


    Range %

    82-96


    1:2

    Average %

    87


    Range %

    80-94


    1:4

    Average %

    90


    Range %

    85-95


    1:8

    Average %

    95


    Range %

    89-101


  • 回收率:

    The recovery of human RELN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    85

    81-92


    EDTA plasma (n=4)

    93

    88-99


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    7

    1.954

    1.834

    1.894

    1.698


    2.33

    1.569

    1.667

    1.618

    1.422


    0.77

    1.035

    1.028

    1.032

    0.836


    0.259

    0.654

    0.629

    0.642

    0.446


    0.086

    0.380

    0.391

    0.386

    0.190


    0.028

    0.312

    0.303

    0.308

    0.111


    0.009

    0.247

    0.254

    0.251

    0.055


    0

    0.197

    0.195

    0.196




  • 货期:
    3-5 working days

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