首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Ras相关蛋白Rab-35(RAB35)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的RAB35含量。RAB35属于RAB蛋白家族成员，在细胞内吞、运输等过程起关键作用。其研究机制主要围绕调节膜泡运输，如参与细胞有丝分裂时胞质分裂。异常的RAB35功能与肿瘤、神经系统疾病等病理过程相关，为相关疾病治疗提供潜在靶点。试剂盒检测范围为47 pg/mL-3000 pg/mL，可用于探究RAB35在肿瘤细胞侵袭转移中的动态变化、神经突触形成相关分子机制，或评估药物干预对特定信号通路中RAB35表达水平的影响，为功能研究与靶点验证提供可靠工具。满足细胞生物学、分子机制研究及疾病模型构建等科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GTP binding protein RAY ELISA Kit; GTP-binding protein RAY ELISA Kit; H ray ELISA Kit; OTTHUMP00000240256 ELISA Kit; OTTHUMP00000240257 ELISA Kit; OTTHUMP00000240258 ELISA Kit; OTTHUMP00000240259 ELISA Kit; RAB1C ELISA Kit; Rab35 ELISA Kit; RAB35 member RAS oncogene family ELISA Kit; RAB35; member RAS oncogene family ELISA Kit; RAB35_HUMAN ELISA Kit; Ras related protein rab 1c (GTP binding protein ray) ELISA Kit; Ras related protein Rab 1C ELISA Kit; Ras related protein Rab35 ELISA Kit; Ras-related protein Rab-1C ELISA Kit; Ras-related protein Rab-35 ELISA Kit; RAY ELISA Kit
缩写：RAB35
Uniprot No.：Q15286
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RAB35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of human RAB35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-102 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.8522.7522.8022.63015002.2002.1002.1501.9787501.3711.2711.3211.1493750.7900.7800.7850.613187.50.5310.5210.5260.354940.4130.4030.4080.236470.2470.2370.2420.07000.1720.1710.172 | ||||||
货期：3-5 working days
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