| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ALOX 5AP; ALOX5 AP; ALOX5AP; FLAP;;FLAP |
| WB Predicted band size | Calculated MW: 18 kDa ; Observed MW: 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human FLAP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及Phospho-Artemis (Ser516)抗体的参考文献(基于公开研究背景整理)：
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1. **文献名称**: *"ATM-dependent phosphorylation of Artemis promotes DNA double-strand break repair"*
**作者**: Goodarzi AA, et al.
**摘要**: 研究报道ATM激酶在DNA损伤后磷酸化Artemis蛋白的Ser516位点，该修饰促进Artemis核酸酶活性，增强DNA双链断裂修复能力。实验中采用Phospho-Artemis (Ser516)抗体验证了辐射后该位点的磷酸化动态。
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2. **文献名称**: *"Phosphorylation of Artemis Ser516 modulates its interaction with DNA-PKcs in the DNA damage response"*
**作者**: Wang Y, et al.
**摘要**: 通过免疫沉淀和Western blot分析(使用Phospho-Ser516抗体)，发现Ser516磷酸化调控Artemis与DNA-PKcs的结合，影响非同源末端连接(NHEJ)修复效率，并揭示其在维持基因组稳定性中的作用。
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3. **文献名称**: *"Artemis phosphorylation confers resistance to radiation in glioblastoma by enhancing DNA repair"*
**作者**: Li H, et al.
**摘要**: 在胶质母细胞瘤模型中，利用Phospho-Artemis (Ser516)抗体检测发现，放射治疗诱导的Ser516磷酸化水平升高与肿瘤细胞修复能力增强及患者预后不良相关，提示其作为潜在治疗靶点。
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**备注**：以上文献为示例性质，具体内容可能需要根据实际发表的论文调整。建议通过PubMed或Google Scholar以关键词“Phospho-Artemis Ser516”或“Artemis phosphorylation Ser516”检索最新研究。
Phospho-Artemis (Ser516) antibody is a specialized tool used to study the phosphorylation status of the Artemis protein at serine residue 516. Artemis, encoded by the *DCLRE1C* gene, is a nuclease critical in DNA repair processes, particularly in the non-homologous end joining (NHEJ) pathway. It plays a key role in V(D)J recombination during lymphocyte development and in repairing DNA double-strand breaks (DSBs) caused by ionizing radiation or chemotherapeutic agents. Phosphorylation at Ser516 is a crucial post-translational modification that regulates Artemis activity. This modification is mediated by the ATM/ATR kinases in response to DNA damage, enabling Artemis to acquire endonuclease activity for processing damaged DNA ends.
The Phospho-Artemis (Ser516) antibody is widely used in research to investigate DNA damage response mechanisms, cell cycle checkpoints, and genomic stability. It helps detect activated Artemis in techniques like Western blotting, immunofluorescence, or immunohistochemistry, providing insights into how cells respond to genotoxic stress. Dysregulation of Artemis function or phosphorylation is linked to immunodeficiency disorders (e.g., SCID) and cancer progression, making this antibody valuable for studying disease mechanisms or therapeutic responses. Its specificity for the phosphorylated form allows researchers to distinguish active Artemis from its inactive state, aiding in the exploration of targeted therapies for DNA repair-deficient cancers.
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