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产品描述：人甲状旁腺激素相关蛋白(PTHrP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTHLH含量。PTHLH即甲状旁腺激素样激素，在胚胎发育、骨骼生长等过程发挥关键作用。其异常表达与多种疾病尤其肿瘤相关。研究聚焦于其与受体结合机制、信号转导通路，以探索通过干预该靶点治疗疾病的新策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，可广泛应用于癌症机制研究、内分泌系统功能探索及骨代谢相关基础科研领域，为体外样本中PTHrP的动态变化提供可靠的检测工具，适用于细胞实验、动物模型或临床前研究中的生物标志物分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HHM ELISA Kit; MGC14611 ELISA Kit; Osteostatin ELISA Kit; Parathyroid hormon like hormone isoform 2 preproprotein ELISA Kit; Parathyroid hormone like hormone ELISA Kit; Parathyroid hormone like hormone isoform 1 preproprotein ELISA Kit; Parathyroid hormone like protein ELISA Kit; Parathyroid hormone like related protein ELISA Kit; Parathyroid hormone related protein ELISA Kit; Parathyroid hormone-like protein ELISA Kit; Parathyroid like protein ELISA Kit; PLP ELISA Kit; PTH related protein ELISA Kit; PTH-rP ELISA Kit; PTHLH ELISA Kit; PTHR ELISA Kit; PTHR_HUMAN ELISA Kit; PTHrP ELISA Kit; PTHrP[107-139] ELISA Kit
缩写：PTHLH
Uniprot No.：P12272
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PTHrP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of human PTHrP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-94 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.4012.4582.4302.33210001.7631.7881.7761.6785001.1841.1951.1901.0922500.8210.8850.8530.7551250.3980.3670.3830.28562.50.2280.2210.2250.12731.250.1060.0990.1030.00500.0950.1010.098 | ||||||
货期：3-5 working days
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