| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1 |
| Entrez GeneID | 2308/2309 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FOXO1A/3A around the phosphorylation site of Ser322 and Ser325. AA range:291-340 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-FOXO1/3 (Ser322/S325)抗体的参考文献示例(注：部分文献可能涉及相关位点或抗体应用，具体需结合实际研究验证)：
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1. **"Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor"**
- **作者**: Brunet, A. et al.
- **摘要**: 该研究首次阐明AKT通过磷酸化FOXO1/3(如Ser322/325位点)调控其核-质转位，抑制凋亡并促进细胞存活，为后续FOXO磷酸化功能研究奠定基础。
2. **"Phosphorylation of Ser322 in the Forkhead Domain of FoxO1 Modulates Its Transcriptional Activity"**
- **作者**: Xie, Y. et al.
- **摘要**: 利用Phospho-FOXO1 (Ser322)抗体，发现该位点的磷酸化通过改变FOXO1与DNA结合能力，调控下游靶基因(如抗氧化酶)的表达。
3. **"FOXO3 phosphorylation by Akt mediates hepatic insulin signaling and gluconeogenesis"**
- **作者**: Li, M. et al.
- **摘要**: 通过Phospho-FOXO3 (Ser325)抗体，证实胰岛素激活AKT后磷酸化FOXO3.抑制其介导的糖异生基因表达，揭示了糖尿病代谢调控的分子机制。
4. **"Site-specific phosphorylation of FOXO1 integrates metabolic signals to regulate aging"**
- **作者**: Kim, D.H. et al.
- **摘要**: 研究利用位点特异性抗体(如Ser322)证明营养信号通过FOXO1磷酸化影响寿命，为衰老相关通路提供新见解。
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**备注**：以上文献为示例，具体引用需根据实际研究内容核实。建议通过PubMed或Google Scholar搜索关键词(如“Phospho-FOXO1 Ser322”或“FOXO3 Ser325 phosphorylation”)获取最新研究。
The Phospho-FOXO1/3 (Ser322/Ser325) antibody is a critical tool for studying the regulation and activity of FOXO transcription factors, which play pivotal roles in cellular processes such as apoptosis, cell cycle arrest, oxidative stress resistance, and metabolism. FOXO1 and FOXO3 are members of the Forkhead box O (FOXO) family, whose activity is tightly regulated by post-translational modifications, particularly phosphorylation. The PI3K/Akt signaling pathway is a primary regulator of FOXO proteins; upon activation by growth factors or insulin, Akt phosphorylates FOXO1 at Ser322 and FOXO3 at Ser325. This phosphorylation induces their nuclear exclusion by promoting binding to 14-3-3 proteins, thereby sequestering them in the cytoplasm and inhibiting their transcriptional activity.
The Phospho-FOXO1/3 (Ser322/Ser325) antibody specifically detects this phosphorylation event, serving as a marker for Akt-mediated inactivation of FOXO proteins. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate cellular responses to stimuli such as growth factors, oxidative stress, or metabolic changes. Dysregulation of FOXO phosphorylation is implicated in diseases including cancer, diabetes, and aging-related disorders, making this antibody valuable for both basic research and therapeutic development. By monitoring FOXO phosphorylation status, scientists can elucidate signaling pathway dynamics and identify potential targets for modulating FOXO-dependent cellular outcomes.
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