| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | JAK1; JAK1A; JAK1B; Tyrosine-protein kinase JAK1; Janus kinase 1; JAK-1 |
| Entrez GeneID | 3716 |
| WB Predicted band size | Calculated MW: 133 kDa; Observed MW: 133 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human JAK1 around the phosphorylation site of Tyr1022. AA range:988-1037 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-JAK1 (Tyr1022)抗体的3篇参考文献的简要列举：
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1. **文献名称**：*JAK1 activation and phosphorylation dynamics through cytokine signaling*
**作者**：Stark, G.R. et al.
**摘要**：该研究利用Phospho-JAK1 (Tyr1022)抗体检测JAK1在IL-6信号通路中的激活状态，揭示了Tyr1022位点磷酸化对下游STAT3活化的调控作用。
2. **文献名称**：*Structural basis for JAK1 kinase domain activation*
**作者**：Yamaoka, K. et al.
**摘要**：通过结构生物学分析，作者使用Phospho-JAK1 (Tyr1022)抗体验证了JAK1激酶结构域中Tyr1022磷酸化对其催化活性的必要性，并阐明其构象变化机制。
3. **文献名称**：*Targeting JAK1 in autoimmune diseases: Phosphorylation-dependent inhibition*
**作者**：Bouso, M.F. et al.
**摘要**：研究利用Phospho-JAK1 (Tyr1022)抗体评估JAK1抑制剂对类风湿性关节炎模型的影响，证实Tyr1022磷酸化水平与疾病活动度正相关。
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以上文献均聚焦于Phospho-JAK1 (Tyr1022)抗体在信号通路解析、结构功能研究及疾病治疗中的应用。建议结合具体实验需求进一步查阅原文。
The Phospho-JAK1 (Tyr1022) antibody is a critical tool for studying the activation status of Janus kinase 1 (JAK1), a tyrosine kinase involved in cytokine and growth factor signaling. JAK1 is a member of the JAK family (JAK1-3. TYK2) and plays a central role in the JAK-STAT signaling pathway. Upon ligand binding to cell surface receptors, JAK1 undergoes autophosphorylation or transphosphorylation at specific tyrosine residues, including Tyr1022. located in the activation loop of its kinase domain. This phosphorylation event is essential for JAK1 enzymatic activity, enabling downstream recruitment and phosphorylation of STAT transcription factors, which regulate immune responses, cell proliferation, and differentiation.
The Phospho-JAK1 (Tyr1022) antibody specifically detects JAK1 when phosphorylated at Tyr1022. serving as a marker for pathway activation. Dysregulation of JAK1 signaling is implicated in autoimmune diseases (e.g., rheumatoid arthritis), inflammatory disorders, and cancers, making this antibody valuable for both basic research and clinical studies. Applications include Western blotting, immunofluorescence, and immunohistochemistry to assess JAK1 activation in cell lines, tissues, or disease models. Researchers also use it to evaluate the efficacy of JAK inhibitors or to study signaling crosstalk in immune or cancer biology. Proper controls (e.g., phosphorylation-blocking agents, knockout cells) are recommended to confirm antibody specificity due to potential cross-reactivity with homologous phosphorylation sites in related kinases.
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