首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔甲状旁腺激素(PTH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PTH含量。甲状旁腺激素（PTH）是甲状旁腺主细胞分泌的肽类激素，主要作用是调节钙磷代谢，维持血钙平衡。研究机制方面，PTH可作用于骨骼、肾脏等靶器官，促进骨钙释放、增加肾小管对钙重吸收等，其异常与多种钙磷代谢相关疾病密切相关。试剂盒检测范围为7.8 pg/mL-500 pg/mL，可应用于内分泌研究、钙代谢调节机制探索、动物疾病模型（如甲状旁腺功能异常）的激素水平评估等科研场景，为实验动物生理状态监测及药物干预效果评价提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PTH
Uniprot No.：G1SNZ6
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 87-92 | |||||
回收率：
| The recovery of rabbit PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 97 | 92-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.9113.0202.9662.8272502.4052.5102.4582.3191251.7561.7721.7641.62562.51.1061.1891.1481.00931.20.5820.6130.5980.45915.60.4900.4570.4740.3357.80.3000.3050.3030.16400.1340.1440.139 | ||||||
货期：3-5 working days
×
