| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ARHGAP35; GRF1; GRLF1; KIAA1722; Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A |
| Entrez GeneID | 2909 |
| WB Predicted band size | Calculated MW: 171 kDa; Observed MW: 190 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human GRF-1 (phospho Tyr1087) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于Phospho-GRLF1 (Tyr1087)抗体的相关文献示例(注：具体位点编号可能存在文献差异，以下为模拟参考内容)：
---
1. **文献名称**: *Tyrosine phosphorylation regulates the activity of RasGRF1 in EGF receptor signaling*
**作者**: Smith A, et al.
**摘要**: 研究揭示了EGF刺激后RasGRF1酪氨酸磷酸化的分子机制，发现Tyr1087位点的磷酸化可增强其鸟苷酸交换因子活性，促进Ras信号通路激活。
2. **文献名称**: *Role of RasGRF1 phosphorylation in neuronal differentiation*
**作者**: Lee J, et al.
**摘要**: 通过抗体验证发现，Tyr1087磷酸化的RasGRF1在PC12细胞分化中起关键作用，其磷酸化水平受NGF调控并与MAPK通路激活相关。
3. **文献名称**: *Characterization of a phospho-specific antibody for RasGRF1 Tyr1087*
**作者**: Chen X, et al.
**摘要**: 该文献报道了一种特异性识别RasGRF1 Tyr1087磷酸化位点的抗体的开发与验证，并应用于检测胰岛素刺激下该位点的动态修饰变化。
4. **文献名称**: *Phosphorylation-dependent interactions of RasGRF1 with SOS1 in cancer cells*
**作者**: Wang H, et al.
**摘要**: 研究利用Phospho-GRLF1 (Tyr1087)抗体证实，该位点的磷酸化促进了RasGRF1与SOS1的结合，进而驱动乳腺癌细胞的增殖和迁移。
---
**备注**：上述文献为示例，实际研究中请通过PubMed或Web of Science以关键词“RasGRF1 phosphorylation Tyr1087”或抗体编号(如CST #XXXXX)检索具体文献。部分抗体说明书可能引用相关研究作为验证依据。
The Phospho-GRLF1 (Tyr1087) antibody is a specialized tool used to detect the phosphorylation status of Guanine Nucleotide Releasing Factor 1 (GRLF1) at tyrosine residue 1087. GRLF1. also known as RasGRF1 or CDC25. is a member of the RasGEF family that activates Ras signaling by promoting the exchange of GDP for GTP. This protein plays a role in regulating cell growth, differentiation, and synaptic plasticity, particularly in neuronal and endocrine tissues. Phosphorylation at Tyr1087 is a critical post-translational modification linked to GRLF1 activation, often triggered by upstream signals such as receptor tyrosine kinases (e.g., EGFR, insulin receptor). This modification enhances its GEF activity, facilitating Ras-MAPK pathway activation and downstream cellular responses.
The Phospho-GRLF1 (Tyr1087) antibody is widely used in biomedical research to study signaling dynamics in conditions like cancer, neurological disorders, and metabolic diseases. It enables specific detection of the activated form of GRLF1 in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Validation typically includes testing in knockout models or phosphorylation-inhibited samples to confirm specificity. Researchers rely on this antibody to explore mechanisms of Ras pathway dysregulation, drug-target interactions, or biomarker discovery, making it essential for understanding GRLF1-related signaling networks in health and disease.
×
