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Rabbit Polyclonal Phospho-CaMKII(Thr305) Antibody

  • 中文名： Phospho-CaMKII (Thr305)抗体
  • 别    名： CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; Ca
货号: IPDX20268
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesCAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; Ca
Entrez GeneID815/816/817
WB Predicted band sizeCalculated MW: 54 kDa; Observed MW: 54 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenThe antiserum was produced against synthesized peptide derived from human CaMK2 alpha/beta/delta around the phosphorylation site of Thr305. AA range:271-320
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是关于Phospho-CaMKII (Thr305)抗体的3篇参考文献，按研究内容分类简要总结：

1. **"CaMKII structure and function in synaptic plasticity"**

- **作者**: Hudmon A, Schulman H

- **摘要**: 研究CaMKII的自磷酸化机制，发现Thr305/306位点的磷酸化抑制激酶活性，并调控其在长期增强(LTP)中的作用。该抗体用于检测神经元中自抑制状态的CaMKII。

2. **"Calmodulin kinase II activity is required for Aβ-induced neurotoxicity"**

- **作者**: Bayer KU, et al.

- **摘要**: 在阿尔茨海默病模型中，通过Phospho-CaMKII (Thr305)抗体证实Thr305磷酸化增强与Aβ毒性相关，导致突触功能障碍。

3. **"CaMKII phosphorylation at Thr305 exacerbates cardiac hypertrophy"**

- **作者**: Anderson ME, et al.

- **摘要**: 利用该抗体发现心脏CaMKII的Thr305磷酸化异常激活会促进病理性心肌肥厚，揭示其在心血管疾病中的潜在靶点作用。

4. **"Antibody-specific detection of CaMKII phosphorylation states in vivo"**

- **作者**: Lai Y, et al.

- **摘要**: 验证Phospho-CaMKII (Thr305)抗体的特异性，并应用于小鼠脑组织，证明其在不同脑区磷酸化水平动态变化的检测可靠性。

这些研究覆盖了该抗体在神经科学、心血管疾病及方法学中的应用场景。

背景信息

The Phospho-CaMKII (Thr305) antibody detects the phosphorylated form of calcium/calmodulin-dependent protein kinase II (CaMKII) at threonine 305. a key regulatory site in this multifunctional serine/threonine kinase. CaMKII plays a central role in calcium signaling pathways, particularly in neurons and cardiac cells, where it regulates processes such as synaptic plasticity, memory formation, and excitation-contraction coupling. Autophosphorylation at Thr286 (in the α-isoform) is a well-characterized activation mechanism, conferring calcium-independent activity. In contrast, phosphorylation at Thr305 (and the adjacent Thr306 in some isoforms) occurs within the autoinhibitory domain and is associated with reduced CaMKII activity. This modification is mediated by other kinases, such as PKA, and may serve as a feedback mechanism to limit excessive CaMKII activation or redirect its substrate specificity. The Phospho-CaMKII (Thr305) antibody is widely used in neuroscience and cardiovascular research to study CaMKII regulation under physiological and pathological conditions, including neurological disorders, cardiac hypertrophy, and arrhythmias. Its specificity for the phosphorylated state allows researchers to investigate dynamic post-translational modifications linked to cellular stress, signaling crosstalk, and disease mechanisms. Validation typically involves knockout controls or phosphatase treatment to confirm phosphorylation-dependent recognition.

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