首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人环加氧酶2(COX-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PTGS2含量。PTGS2，也称为COX-2，是一种在炎症和组织损伤时表达升高的酶，参与前列腺素生物合成，与炎症反应、细胞凋亡等多种生理和病理过程密切相关。其研究机制涉及基因表达调控、蛋白相互作用等，在疾病如肿瘤、炎症性疾病、心血管疾病中发挥重要作用。试剂盒检测范围为1.25 ng/mL-80 ng/mL，该产品可广泛用于细胞模型中炎症因子诱导实验、动物疾病模型组织分析、肿瘤微环境研究以及抗炎/抗肿瘤药物体外筛选等场景，为探究COX - 2在病理生理过程中的调控机制、评估药物对COX - 2通路的抑制作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：COX 2 ELISA Kit; COX-2 ELISA Kit; COX2 ELISA Kit; Cyclooxygenase 2 ELISA Kit; Cyclooxygenase 2b ELISA Kit; Cyclooxygenase ELISA Kit; Cyclooxygenase-2 ELISA Kit; Cyclooxygenase2 ELISA Kit; EC 1.14.99.1 ELISA Kit; fj02a10 ELISA Kit; Glucocorticoid-regulated inflammatory cyclooxygenase ELISA Kit; Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase ELISA Kit; GRIPGHS ELISA Kit; hCox 2 ELISA Kit; Macrophage activation-associated marker protein P71/73 ELISA Kit; OTTHUMP00000033524 ELISA Kit; PES-2 ELISA Kit; PGG/HS ELISA Kit; PGH synthase 2 ELISA Kit; PGH2_HUMAN ELISA Kit; PGHS 2 ELISA Kit; PGHS-2 ELISA Kit; PGHS2 ELISA Kit; PHS 2 ELISA Kit; PHS II ELISA Kit; PHS2 ELISA Kit; Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) ELISA Kit; Prostaglandin endoperoxide synthase 2 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; Prostaglandin G/H synthase 2 precursor ELISA Kit; Prostaglandin G/H synthase and cyclooxygenase ELISA Kit; Prostaglandin G/H synthase ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit; PTGS2 ELISA Kit; ptgs2a ELISA Kit; TIS10 ELISA Kit; TIS10 protein ELISA Kit; unp1239 ELISA Kit; wu:fj02a10 ELISA Kit
缩写：PTGS2
Uniprot No.：P35354
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 87-100 | ||||
| EDTA plasma (n=4) | 88 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.7012.6522.6772.583402.0921.9492.0211.927201.3011.2281.2651.171100.5870.5710.5790.48550.3570.3740.3660.2722.50.2070.2130.2100.1161.250.1520.1610.1570.06300.0920.0960.094 | ||||||
货期：3-5 working days
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