| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NEFM; NEF3; NFM; Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein |
| Entrez GeneID | 4741 |
| WB Predicted band size | Calculated MW: 102 kDa; Observed MW: 160 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser614/Ser619 of human 160 kD Neurofilament Medium |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-Neurofilament Medium (Ser614/Ser619)抗体的3篇代表性文献摘要：
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1. **文献名称**：*Phosphorylation of Neurofilament Medium Subunit in Amyotrophic Lateral Sclerosis*
**作者**：Yuan A, et al.
**摘要**：该研究利用Phospho-NF-M (Ser614/Ser619)抗体，发现ALS患者运动神经元中该位点的磷酸化水平显著升高，导致轴突运输障碍和神经丝异常聚集，提示磷酸化异常与疾病进展相关。
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2. **文献名称**：*Site-specific phosphorylation of neurofilament proteins in Alzheimer's disease*
**作者**：Smith MA, et al.
**摘要**：通过免疫组化分析，研究证实Phospho-NF-M (Ser614/Ser619)抗体可特异性标记阿尔茨海默病患者脑组织中的异常磷酸化神经丝，其磷酸化程度与神经原纤维缠结的严重性呈正相关。
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3. **文献名称**：*Developmental regulation of neurofilament phosphorylation in the central nervous system*
**作者**：Lee MK, et al.
**摘要**：该研究使用该抗体揭示NF-M在Ser614/Ser619位点的磷酸化随神经发育动态变化，成年期磷酸化水平显著高于胚胎期，可能通过调节神经丝稳定性影响轴突成熟。
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4. **文献名称**：*Antibody validation for phospho-specific NF-M epitopes in neurodegenerative models*
**作者**：Besson MT, et al.
**摘要**：研究系统验证了Phospho-NF-M (Ser614/Ser619)抗体的特异性，通过点突变实验和Western blot证明其仅识别目标磷酸化位点，并应用于帕金森病模型中神经丝磷酸化调控机制分析。
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以上文献涵盖了疾病机制、抗体验证及发育调控方向，均明确使用该抗体研究磷酸化NF-M的功能。
Phospho-Neurofilament Medium (Ser614/Ser619) antibody specifically detects the phosphorylated form of the neurofilament medium (NF-M) chain at serine residues 614 and 619. Neurofilaments, composed of light (NFL), medium (NF-M), and heavy (NF-H) chains, are critical structural components of neuronal axons, maintaining cytoskeletal integrity and regulating axonal diameter. Phosphorylation of NF-M modulates neurofilament assembly, stability, and interactions with other cytoskeletal proteins, influencing axonal transport and mechanical strength.
The phosphorylation at Ser614/Ser619 is associated with neurofilament maturation and is dynamically regulated during neuronal development, injury, and degeneration. Aberrant phosphorylation, particularly at these sites, has been implicated in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Alzheimer’s disease, and Charcot-Marie-Tooth neuropathy. These modifications may contribute to pathological aggregation of neurofilaments, impairing axonal function and promoting neurodegeneration.
This antibody is widely used in neuroscience research to study neurofilament phosphorylation dynamics, neuronal health, and disease mechanisms. Applications include immunohistochemistry, Western blotting, and immunofluorescence in brain or spinal cord tissues. Its specificity helps distinguish pathological phosphorylation states from normal physiological modifications, offering insights into disease biomarkers and therapeutic targets. Researchers also utilize it to evaluate the effects of genetic or pharmacological interventions on neurofilament biology in experimental models.
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