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产品描述:人26S蛋白酶体非ATP酶调节亚基4(PSMD4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PSMD4含量。PSMD4在细胞内发挥重要作用。它是蛋白酶体盖子的组成部分,参与细胞内蛋白质降解等关键生理过程。研究发现其与肿瘤发生发展密切相关,可能通过调节致癌蛋白的降解来影响肿瘤细胞生长、增殖、凋亡等,是潜在的癌症治疗靶点。试剂盒检测范围为34.4 pg/mL-2200 pg/mL,适用于基础科研中蛋白酶体功能研究、肿瘤发生机制探索、神经退行性疾病模型分析以及药物干预对泛素化降解通路影响的评估,为研究蛋白质稳态调控提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:26S protease subunit S5a ELISA Kit; 26S proteasome non ATPase regulatory subunit 4 ELISA Kit; 26S proteasome non-ATPase regulatory subunit 4 ELISA Kit; 26S proteasome regulatory subunit rpn10 ELISA Kit; 26S proteasome regulatory subunit S5A ELISA Kit; AF 1 ELISA Kit; AF ELISA Kit; AF1 ELISA Kit; Angiocidin ELISA Kit; Antisecretory factor 1 ELISA Kit; ASF ELISA Kit; DS5a ELISA Kit; MCB 1 ELISA Kit; MCB1 ELISA Kit; Multiubiquitin chain binding protein ELISA Kit; Multiubiquitin chain-binding protein ELISA Kit; OTTHUMP00000059963 ELISA Kit; Prosome macropain ELISA Kit; Proteasome (prosome macropain) 26S subunit non ATPase 4 ELISA Kit; Proteasome 19S S5A ELISA Kit; Proteasome 26S non ATPase subunit 4 ELISA Kit; Proteasome 26S subunit non ATPase 4 ELISA Kit; PSMD 4 ELISA Kit; Psmd4 ELISA Kit; PSMD4_HUMAN ELISA Kit; pUB R5 ELISA Kit; pUBR5 ELISA Kit; Rpn 10 ELISA Kit; Rpn10 ELISA Kit; RPN10 homolog ELISA Kit; S5A ELISA Kit; S5a/antisecretory factor protein ELISA Kit
缩写:PSMD4
Uniprot No.:P55036
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:34.4 pg/mL-2200 pg/mL
灵敏度:8.6 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human PSMD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 90 | |||||
Range % | 84-94 | ||||||
1:2 | Average % | 102 | |||||
Range % | 97-107 | ||||||
1:4 | Average % | 98 | |||||
Range % | 88-102 | ||||||
1:8 | Average % | 99 | |||||
Range % | 92-104 |
回收率:
The recovery of human PSMD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 98 | 91-102 | |||||
EDTA plasma (n=4) | 95 | 88-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected 22002.3022.2342.2682.120 11001.5601.4881.5241.376 5500.9010.9320.9170.769 2750.5020.5110.5070.359 137.50.2930.2870.2900.142 68.80.2490.2460.2480.100 34.40.1960.1900.1930.045 00.1520.1440.148 |
货期:3-5 working days
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