| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RPS6KA5; MSK1; Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL |
| Entrez GeneID | 9252 |
| WB Predicted band size | Calculated MW: 90 kDa; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human Phospho-MSK1 (S376) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-MSK1 (Ser376)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
1. **文献名称**：*"MSK1 regulates homeostatic and experience-dependent synaptic plasticity"*
**作者**：Ma X, et al.
**摘要**：该研究通过Phospho-MSK1 (Ser376)抗体检测神经元中MSK1的磷酸化状态，发现MSK1在突触可塑性和记忆形成中起关键作用，其Ser376位点的磷酸化响应于神经元活动，调控下游CREB信号通路。
2. **文献名称**：*"p38 MAPK/MSK1 signaling modulates histone H3 phosphorylation and gene expression in response to oxidative stress"*
**作者**：Soloaga A, et al.
**摘要**：研究利用Phospho-MSK1 (Ser376)抗体证实，氧化应激通过p38 MAPK激活MSK1.导致Ser376位点磷酸化，进而磷酸化组蛋白H3.促进抗氧化基因的转录激活。
3. **文献名称**：*"Phosphorylation of MSK1 at Ser376 links ERK signaling to chromatin remodeling in cancer cells"*
**作者**：McCoy CE, et al.
**摘要**：文章发现，在癌细胞中，ERK通路通过磷酸化MSK1的Ser376位点驱动其核转位，进而介导染色质重塑和促增殖基因的表达，该过程通过特异性抗体验证了磷酸化修饰的功能意义。
以上文献均围绕Phospho-MSK1 (Ser376)的生物学功能展开，涵盖神经科学、氧化应激响应及癌症信号转导等领域，抗体应用聚焦于机制验证和功能研究。
The Phospho-MSK1 (Ser376) antibody is a specialized tool used to detect the activated form of mitogen- and stress-activated protein kinase 1 (MSK1), specifically phosphorylated at serine residue 376. MSK1. a serine/threonine kinase downstream of the MAPK (mitogen-activated protein kinase) signaling pathway, plays a critical role in regulating gene expression by phosphorylating transcription factors such as CREB and NF-κB. Its activation involves dual phosphorylation: first by ERK or p38 MAPK at Thr581/Thr700. and subsequently by autophosphorylation at Ser376 within the C-terminal regulatory domain. The phosphorylation at Ser376 is essential for MSK1's full enzymatic activity and nuclear translocation, linking extracellular stimuli (e.g., stress, growth factors) to transcriptional responses.
This antibody is widely used in research to study MSK1 activation dynamics in cellular processes like inflammation, proliferation, and apoptosis. It enables the detection of phosphorylated MSK1 via techniques such as Western blotting, immunofluorescence, or immunoprecipitation, providing insights into pathway activity in disease models (e.g., cancer, immune disorders). Validation typically includes testing in knockout cells or phosphatase-treated lysates to confirm specificity. Researchers utilize it to explore MSK1's role in signaling crosstalk, drug response, or as a potential therapeutic target.
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