| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ATF2; CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding pro |
| Entrez GeneID | 1386 |
| WB Predicted band size | Calculated MW: 55 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Phospho-ATF2 (T71) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于Phospho-ATF2 (Thr71)抗体的3篇示例参考文献(内容为模拟概括，非真实文献)：
1. **文献名称**：*"ATF2 phosphorylation at Thr71 mediates oxidative stress-induced apoptosis in neurons"*
**作者**：Chen et al.
**摘要**：研究揭示ATF2在氧化应激下通过Thr71位点的磷酸化激活其促凋亡功能，该抗体用于验证神经元中ATF2的磷酸化水平与凋亡信号通路的关系。
2. **文献名称**：*"p38 MAPK-dependent phosphorylation of ATF2 on Thr71 regulates IL-6 gene expression in macrophages"*
**作者**：Kumar & Yamamoto
**摘要**：通过体外实验证明p38 MAPK通路依赖的ATF2 Thr71磷酸化调控巨噬细胞IL-6的分泌，利用该抗体进行免疫印迹和染色质免疫沉淀分析。
3. **文献名称**：*"Phosphorylation of ATF2 at Thr71 enhances DNA repair by promoting interaction with BRCA1"*
**作者**：Rodriguez et al.
**摘要**：发现ATF2 Thr71磷酸化在DNA损伤修复中通过与BRCA1蛋白结合发挥作用，该抗体用于免疫荧光定位及蛋白质相互作用研究。
4. **文献名称**：*"Targeting ATF2 phosphorylation in melanoma: Thr71 as a biomarker for therapeutic resistance"*
**作者**：Lee et al.
**摘要**：分析黑色素瘤中Phospho-ATF2 (Thr71)的表达与靶向治疗耐药性的相关性，该抗体用于临床样本的免疫组化评估。
(注：以上文献为示例性内容，实际引用需查询真实数据库如PubMed或Web of Science。)
The Phospho-ATF2 (Thr71) antibody is a specialized tool used to detect the activated form of Activating Transcription Factor 2 (ATF2), a member of the leucine zipper family of DNA-binding proteins. ATF2 plays a critical role in cellular stress responses, apoptosis, and proliferation by regulating the transcription of target genes. Its activity is tightly controlled by post-translational modifications, particularly phosphorylation. The Thr71 residue, located within the transactivation domain of ATF2. is phosphorylated by stress-activated kinases such as JNK (c-Jun N-terminal kinase) and p38 MAPK (mitogen-activated protein kinase) in response to DNA damage, cytokines, or oxidative stress. This phosphorylation event enhances ATF2's transcriptional activity by promoting dimerization and DNA binding.
The Phospho-ATF2 (Thr71) antibody specifically recognizes ATF2 when phosphorylated at Thr71. enabling researchers to study its activation status in various experimental models. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate signaling pathways involving stress responses, inflammation, or oncogenesis. Dysregulation of ATF2 phosphorylation has been implicated in cancer progression, neurodegenerative diseases, and immune disorders, making this antibody valuable for both mechanistic studies and therapeutic target validation. Its specificity and reliability depend on proper validation, including knockout controls and peptide competition assays.
×
