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产品描述：人蛋白酶体（prosome，macropain）亚基，β型，2(PSMB2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PSMB2含量。PSMB2 是一个重要靶点。它在多种生理和病理过程中发挥作用，可能与细胞内蛋白降解、免疫调节等机制相关。对其研究有助于深入了解疾病发生发展的分子机制，为开发针对该靶点的药物，治疗相关疾病提供理论依据。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础科研中探究蛋白酶体功能调控机制、肿瘤发生发展相关蛋白代谢异常、神经退行性疾病病理模型中的蛋白稳态失衡等研究领域；可高效支持体外培养细胞、动物模型组织或体液样本中PSMB2的定量分析，为研究蛋白质降解通路在疾病发生中的作用或药物干预效果评价提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HC7 I ELISA Kit; Macropain subunit C7 I ELISA Kit; Macropain subunit C7-I ELISA Kit; Multicatalytic endopeptidase complex subunit C7 1 ELISA Kit; Multicatalytic endopeptidase complex subunit C7 I ELISA Kit; Multicatalytic endopeptidase complex subunit C7-I ELISA Kit; Proteasome (prosome, macropain) subunit beta type 2 ELISA Kit; Proteasome beta 2 subunit ELISA Kit; Proteasome component C7 I ELISA Kit; Proteasome component C7-I ELISA Kit; Proteasome subunit beta type-2 ELISA Kit; PSB2_HUMAN ELISA Kit; Psmb2 ELISA Kit
缩写：PSMB2
Uniprot No.：P49721
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.2 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PSMB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 104 | ||||
| Range % | 99-108 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-97 | |||||
回收率：
| The recovery of human PSMB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.7502.8522.8012.6568002.0482.1472.0981.9534001.2851.3831.3341.1892000.7630.7850.7740.6291000.4160.4240.4200.275500.2510.2620.2570.112250.2040.2100.2070.06200.1440.1450.145 | ||||||
货期：3-5 working days
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