| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CREB; CREB-1 |
| Entrez GeneID | 1385 |
| clone | 3C4 |
| WB Predicted band size | Calculated MW: 35 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇关于Phospho-CREB (Ser133)抗体的经典参考文献，内容基于真实研究概括：
---
1. **"Phosphorylated CREB binds specifically to the nuclear protein CBP"**
*作者：Chrivia et al. (1993)*
**摘要**：首次证明CREB在Ser133位点磷酸化后与转录共激活因子CBP结合，揭示了cAMP信号通路中转录激活的分子机制。
2. **"Regulation of CREB phosphorylation in the suprachiasmatic nucleus by light and circadian clock"**
*作者：Ginty et al. (1993)*
**摘要**：通过Phospho-CREB (Ser133)抗体检测昼夜节律调控中CREB的磷酸化状态，发现光刺激通过MAPK通路激活CREB。
3. **"Transcriptional regulation by the phosphorylation-dependent factor CREB"**
*作者：Mayr & Montminy (2001)*
**摘要**：综述CREB的磷酸化机制及其在基因表达调控中的作用，强调Ser133位点磷酸化抗体的应用价值。
---
以上文献涵盖了CREB磷酸化的机制、功能及实验检测方法，均为该领域奠基性研究。
Phospho-CREB (Ser133) antibodies are essential tools for studying the activation status of cAMP response element-binding protein (CREB), a transcription factor central to numerous cellular processes, including synaptic plasticity, memory formation, and cell survival. CREB becomes functionally active upon phosphorylation at serine 133 (Ser133), a modification that enables its interaction with transcriptional coactivators like CBP/p300. This phosphorylation event is primarily mediated by kinases such as protein kinase A (PKA), mitogen-activated protein kinase (MAPK), or calcium/calmodulin-dependent kinases (CaMKs), often in response to extracellular signals like hormones, growth factors, or neurotransmitters.
Phospho-specific antibodies targeting Ser133 allow researchers to detect and quantify the activated form of CREB in various experimental settings, including Western blotting, immunohistochemistry, and flow cytometry. These antibodies are particularly valuable in neuroscience, cancer biology, and metabolic research, where dysregulated CREB activity is linked to diseases like Alzheimer’s, cancer, and diabetes. For instance, elevated phospho-CREB levels are observed in certain tumors, correlating with enhanced cell proliferation and survival.
When using these antibodies, proper sample preparation is critical to preserve phosphorylation signals, as phosphatases in lysates can rapidly dephosphorylate CREB. Controls, such as unstimulated samples or kinase inhibitors, are recommended to validate specificity. Overall, phospho-CREB (Ser133) antibodies serve as indispensable probes for unraveling CREB-related signaling dynamics in health and disease.
×
