首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人前心钠肽(Pro-ANP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Pro-ANP含量。Pro-ANP（前心钠素原）是一个具有重要生理意义的靶点。它可反映心脏功能状态，与心血管系统疾病密切相关。研究机制上，主要聚焦于其在调节体液平衡、血压稳定等方面的作用，探寻其在疾病发生发展中的变化，为相关疾病治疗提供理论依据。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品广泛应用于心血管疾病机制研究、药物干预效果评估、动物模型心脏功能分析等科研场景，尤其适用于探索心力衰竭、高血压及相关代谢性疾病中Pro - ANP的动态变化规律，为实验室提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Pro-ANP
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 86-97 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 96-108 | ||||||
回收率：
| The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-95 | |||||
| EDTA plasma (n=4) | 87 | 82-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40001.9431.8961.9201.76620001.4521.3661.4091.25510000.9980.9680.9830.8295000.6460.6570.6520.4982500.4670.4540.4610.3071250.3220.3060.3140.16062.50.2090.2030.2060.05200.1550.1530.154 | |||||||
货期：3-5 working days
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