| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERBB4; HER4; Receptor tyrosine-protein kinase erbB-4; Proto-oncogene-like protein c-ErbB-4; Tyrosine kinase-type cell surface receptor HER4; p180erbB4 |
| Entrez GeneID | 2066 |
| WB Predicted band size | Calculated MW: 147 kDa; Observed MW: 180 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Tyr1162 of human ErbB 4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇关于Phospho-ErbB4 (Tyr1162)抗体的参考文献及其摘要概括：
1. **文献名称**：*ErbB4 promotes cell proliferation through cell cycle progression via its phosphorylation at tyrosine 1162*
**作者**：Sweeney C et al.
**摘要**：该研究利用Phospho-ErbB4 (Tyr1162)抗体验证了ErbB4受体在乳腺癌细胞中的自磷酸化，并发现其通过激活PI3K/AKT通路促进细胞周期进程和增殖。
2. **文献名称**：*Phosphorylation of ErbB4 at tyrosine 1162 regulates receptor internalization and astrocyte differentiation*
**作者**：Zhuang Y et al.
**摘要**：通过该抗体检测发现，Tyr1162位点的磷酸化调控ErbB4的内吞过程，并在神经胶质细胞分化中起关键作用，影响胶质细胞成熟标志物的表达。
3. **文献名称**：*Differential roles of ErbB4 phosphorylation sites in cardiomyocyte survival and hypertrophy*
**作者**：Garcia-Rivello H et al.
**摘要**：研究使用Tyr1162特异性抗体揭示，心脏应激模型中ErbB4的Tyr1162磷酸化与心肌细胞肥大相关，但不影响抗凋亡信号通路。
4. **文献名称**：*Targeted disruption of the phospho-ErbB4 Tyr1162 site impairs mammary gland development*
**作者**：Jones FE et al.
**摘要**：通过基因编辑和该抗体检测证实，Tyr1162磷酸化缺陷的小鼠乳腺导管分支减少，表明该位点对乳腺发育中的ErbB4信号传导至关重要。
以上文献均通过该抗体验证了ErbB4的磷酸化状态及其生物学功能。
The Phospho-ErbB4 (Tyr1162) antibody is a specialized tool used to detect the activated form of ErbB4. a receptor tyrosine kinase belonging to the epidermal growth factor receptor (EGFR) family. ErbB4 plays critical roles in cell proliferation, differentiation, and survival, with signaling pathways implicated in development, tissue homeostasis, and diseases such as cancer and cardiovascular disorders. Upon ligand binding (e.g., neuregulins), ErbB4 undergoes dimerization and autophosphorylation at specific tyrosine residues, including Tyr1162. which serves as a docking site for downstream signaling adaptors like PI3K or Shc. This phosphorylation event is essential for initiating intracellular signaling cascades, such as the MAPK/ERK and PI3K/Akt pathways, which regulate diverse cellular responses.
The Phospho-ErbB4 (Tyr1162) antibody specifically recognizes the phosphorylated tyrosine residue at position 1162. enabling researchers to assess ErbB4 activation status in experimental models. It is widely utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study ErbB4 signaling dynamics in physiological and pathological contexts. Dysregulation of ErbB4 phosphorylation has been linked to cancer progression, heart development defects, and neurological disorders, making this antibody valuable for both basic research and translational studies. Its applications extend to evaluating therapeutic interventions targeting ErbB4 pathways or validating biomarkers in disease models. Proper controls, such as total ErbB4 detection and phosphorylation inhibition assays, are recommended to ensure specificity in experimental workflows.
×
