首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人主要朊病毒蛋白(PRNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PRNP含量。PRNP基因编码朊蛋白，在神经系统发育及维持中起重要作用。其突变与人类多种神经退行性疾病如克 - 雅病等相关。研究聚焦于朊蛋白错误折叠、聚集机制，以及其在神经元损伤、神经毒性和疾病传播中的作用，为相关疾病防治提供方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于神经退行性疾病机制研究，例如克雅氏病动物模型脑组织内PRNP表达动态分析、细胞培养体系中PrP蛋白水平监测，或探究PrP^C与PrP^Sc相互作用的分子调控网络，为朊病毒相关病理研究及药物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alternative prion protein, major prion protein ELISA Kit; AltPrP ELISA Kit; ASCR ELISA Kit; CD230 ELISA Kit; CD230 antigen ELISA Kit; CJD ELISA Kit; GSS ELISA Kit; KURU ELISA Kit; Major prion protein ELISA Kit; p27 30 ELISA Kit; PRIO_HUMAN ELISA Kit; Prion protein ELISA Kit; Prion related protein ELISA Kit; PRIP ELISA Kit; PRNP ELISA Kit; PrP ELISA Kit; PrP27 30 ELISA Kit; PrP27-30 ELISA Kit; PrP33-35C ELISA Kit; PrPC ELISA Kit; PrPSc ELISA Kit; Sinc ELISA Kit
缩写：PRNP
Uniprot No.：P04156
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||||||||
| Sample | Serum(n=4) | |||||||||||
| 1:1 | Average % | 87 | ||||||||||
| Range % | 81-90 | |||||||||||
| 1:2 | Average % | 100 | ||||||||||
| Range % | 96-104 | |||||||||||
| 1:4 | Average % | 93 | ||||||||||
| Range % | 87-97 | |||||||||||
| 1:8 | Average % | 98 | ||||||||||
| Range % | 91-103 | |||||||||||
回收率：
| The recovery of human PRNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||||||||
| Sample Type | Average % Recovery | Range | ||||||||||
| Serum (n=5) | 94 | 88-97 | ||||||||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||
| ng/mlOD1OD2AverageCorrected202.5022.4442.4732.331102.0892.0242.0571.91551.4261.4111.4191.2772.50.9300.9390.9350.7931.250.5880.5680.5780.4360.6250.3670.3550.3610.2190.3120.2810.2870.2840.14200.1400.1440.142 | ||||||||||||
货期：3-5 working days
×
