首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人鱼精蛋白1(PRM1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PRM1含量。人鱼精蛋白1（PRM1）是一种参与DNA包装和精子细胞发育的蛋白，位于细胞质和核质中。其研究机制涉及DNA结合活性，可能参与精子变形期与成熟期的蛋白，与精子形态密切相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于探究PRM1在生殖系统生理/病理条件下的表达调控，如精子发生机制研究、环境因素或药物对精子蛋白的影响评估等基础科研领域，亦可为男性生殖健康相关分子标志物研究提供技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PRM1Sperm protamine P1 ELISA Kit; Cysteine-rich protamine ELISA Kit
缩写：PRM1
Uniprot No.：P04553
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-102 | |||||
回收率：
| The recovery of human PRM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-104 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7862.7522.7692.61310002.3062.3762.3412.1855001.6481.6541.6511.4952500.9851.0100.9980.8421250.6770.6640.6710.51562.50.5030.5090.5060.35031.250.2750.2840.2800.12400.1540.1570.156 | ||||||
货期：3-5 working days
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