| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PEPC, PGII |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human PGC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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1. **"Activation of the Double-Stranded RNA-Dependent Protein Kinase PKR by Dengue Virus Infection"**
- 作者：García MA, et al.
- 摘要：研究登革病毒感染如何通过磷酸化PKR(Thr451位点)激活其激酶活性，抑制病毒复制并触发宿主细胞凋亡。
2. **"Phosphorylation of PKR at Thr451 Contributes to Aβ-Induced Neurotoxicity in Alzheimer’s Disease Models"**
- 作者：Bando Y, et al.
- 摘要：探讨β淀粉样蛋白(Aβ)通过诱导PKR Thr451磷酸化激活下游凋亡通路，促进阿尔茨海默病神经元死亡。
3. **"PKR Thr451 Phosphorylation as a Biomarker for Chemotherapeutic Response in Breast Cancer"**
- 作者：Kim SH, et al.
- 摘要：验证Phospho-PKR (Thr451)抗体在乳腺癌组织中的检测应用，发现化疗药物通过该位点磷酸化激活PKR并诱导癌细胞凋亡。
4. **"A Protocol for Specific Detection of Phospho-PKR (Thr451) in Virus-Infected Cells"**
- 作者：Pindel A, Koromilas AE
- 摘要：提供使用Phospho-PKR (Thr451)抗体的实验方法，包括Western blot和免疫荧光技术，验证其在病毒感染模型中的特异性。
Phospho-PKR (Thr451) antibody is a specialized tool used to detect the activated form of Protein Kinase R (PKR), a serine/threonine kinase central to cellular stress responses. PKR, encoded by the *EIF2AK2* gene, is activated by double-stranded RNA (dsRNA) during viral infections or cellular stress. Its activation involves autophosphorylation at specific residues, including Thr451. which is critical for its kinase activity. Once activated, PKR phosphorylates eukaryotic initiation factor 2α (eIF2α), leading to global translational arrest and stress granule formation, thereby modulating antiviral defense, apoptosis, and inflammatory signaling.
The Phospho-PKR (Thr451) antibody specifically recognizes PKR when phosphorylated at Thr451. serving as a marker for its activation status. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to study PKR’s role in viral pathogenesis (e.g., HIV, hepatitis), neurodegenerative diseases, or cancer, where dysregulated PKR signaling is implicated. It helps elucidate mechanisms linking cellular stress to immune responses or programmed cell death. Validation often includes testing in PKR-deficient models or under dsRNA-mimicking conditions (e.g., poly(I:C) treatment) to confirm specificity. This antibody is vital for exploring PKR’s dual role as a pro-survival or pro-apoptotic factor, depending on cellular context and stress duration.
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