首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠蛋白聚糖4(PRG4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PRG4含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，科研人员可将其应用于大鼠关节炎模型构建、软骨退行性病变机制探索、关节液生物标志物分析等领域，也可用于评估运动损伤或药物治疗对关节内PRG4表达的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Plppr2; Lppr2; Prg4; Phospholipid phosphatase-related protein type 2; Lipid phosphate phosphatase-related protein type 2; Plasticity-related gene 4 protein; PRG-4
缩写：PRG4
Uniprot No.：Q6W5G4
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 99-103 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-93 | |||||
回收率：
| The recovery of rat PRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 101 | 98-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.7212.8882.8052.610501.8061.7651.7861.591251.1541.1401.1470.95212.50.7020.6780.6900.4956.250.5120.5200.5160.3213.120.3680.3620.3650.1701.560.2640.2560.2600.06500.1920.1980.195 | ||||||
货期：3-5 working days
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