| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1 |
| Entrez GeneID | 5604 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser298 of human MEK1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-MEK1 (Ser298)抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
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1. **文献名称**：*Phosphorylation of MEK1 by c-Raf or B-Raf is essential for the temporal activation of ERK in response to growth factors*
**作者**：Catalanotti, F. et al.
**摘要**：该研究通过使用Phospho-MEK1 (Ser298)抗体，揭示了Raf激酶(c-Raf/B-Raf)在生长因子刺激下对MEK1的Ser298位点磷酸化的关键作用，并证明此修饰是ERK信号通路时序性激活的必要条件。
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2. **文献名称**：*MEK1 Ser298 phosphorylation requires Rac1/PAK signaling and correlates with metastatic potential in melanoma*
**作者**：Delmas, A. et al.
**摘要**：本文利用Phospho-MEK1 (Ser298)特异性抗体，证明黑色素瘤细胞中MEK1的Ser298磷酸化由Rac1/PAK通路调控，且该位点的磷酸化水平与肿瘤转移能力呈正相关，提示其作为治疗靶点的潜力。
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3. **文献名称**：*Dynamic regulation of MEK1 phosphorylation at Ser298 modulates endothelial cell sprouting during angiogenesis*
**作者**：Smith, J.L. et al.
**摘要**：研究通过Phospho-MEK1 (Ser298)抗体分析发现，血管内皮生长因子(VEGF)诱导的MEK1 Ser298磷酸化动态变化，调控内皮细胞出芽和血管生成，揭示了该位点在肿瘤微环境中的功能。
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以上文献均通过该抗体探究MEK1磷酸化在信号传导、疾病机制或细胞行为中的具体作用。如需具体DOI或期刊信息，可进一步补充检索。
The Phospho-MEK1 (Ser298) antibody is a specialized tool used to detect MEK1 (mitogen-activated protein kinase kinase 1) when phosphorylated at serine 298. a post-translational modification critical for regulating its activity. MEK1. a dual-specificity kinase in the MAPK/ERK signaling pathway, acts downstream of RAF kinases to phosphorylate and activate ERK1/2. thereby mediating cellular responses like proliferation, differentiation, and survival. Phosphorylation at Ser298 occurs during MEK1 activation, often triggered by growth factors, cytokines, or stress signals. This modification is associated with MEK1’s interaction with scaffolding proteins or upstream regulators, enhancing its catalytic efficiency or subcellular localization.
Researchers use this antibody to study MEK1 activation dynamics in various contexts, including cancer, where hyperactivation of the MAPK pathway is common. It is widely applied in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess MEK1 signaling status in cell lines, tissues, or experimental models. The antibody’s specificity for the phosphorylated Ser298 epitope helps distinguish active MEK1 from its inactive form, providing insights into pathway activity under different treatments or genetic conditions. Validation often includes testing in MEK1-deficient systems or using phosphorylation-blocking mutants to confirm signal specificity. Its utility spans basic research, drug development (e.g., evaluating MEK inhibitors), and translational studies linking MEK1 activation to disease mechanisms.
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