| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 |
| Entrez GeneID | 1956 |
| WB Predicted band size | Calculated MW: 134 kDa; Observed MW: 175 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Tyr1068 of human EGFR |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **"EGFR mutation and resistance of non-small-cell lung cancer to gefitinib"** by Lynch et al.
*摘要：研究EGFR酪氨酸激酶抑制剂耐药机制，发现Tyr1068磷酸化状态与EGFR突变导致的信号持续激活相关，涉及AKT/mTOR通路调控。*
2. **"Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib"** by Paez et al.
*摘要：通过Western blot分析Tyr1068磷酸化水平，揭示EGFR突变型肿瘤对吉非替尼敏感性的分子基础，证实磷酸化状态作为治疗响应标志物的潜力。*
3. **"Phosphorylated EGFR expression predicts poor prognosis in colorectal cancer"** by McKay et al.
*摘要：采用Phospho-EGFR (Tyr1068)抗体进行免疫组化分析，发现结直肠癌中EGFR Tyr1068高磷酸化与患者总生存期缩短显著相关，提示其作为预后生物标志物的价值。*
4. **"Targeting the PI3K/AKT/mTOR pathway in EGFR-mutant lung cancer"** by Engelman et al.
*摘要：通过检测Tyr1068磷酸化程度，阐明EGFR与PI3K/AKT通路交叉对话机制，提出联合抑制EGFR和mTOR可克服酪氨酸激酶抑制剂耐药性的治疗策略。*
The Phospho-EGFR (Tyr1068) antibody is a critical tool for studying the activation status of the epidermal growth factor receptor (EGFR), a transmembrane tyrosine kinase receptor involved in regulating cell proliferation, survival, and differentiation. EGFR activation occurs upon ligand binding (e.g., EGF, TGF-α), triggering receptor dimerization and autophosphorylation at specific tyrosine residues, including Tyr1068. This phosphorylation event serves as a docking site for adaptor proteins like Grb2. initiating downstream signaling cascades such as RAS/MAPK and PI3K/AKT pathways, which drive oncogenic processes when dysregulated.
The Phospho-EGFR (Tyr1068) antibody specifically detects EGFR phosphorylated at Tyr1068. making it invaluable for research on cancers with EGFR overexpression or mutations (e.g., non-small cell lung cancer, glioblastoma). It is widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess EGFR activation in response to stimuli or targeted therapies. In clinical contexts, its expression may correlate with disease progression, therapeutic resistance, or sensitivity to EGFR inhibitors like gefitinib or osimertinib. However, interpretation requires caution, as phosphorylation levels can vary with sample handling and experimental conditions. Overall, this antibody bridges basic research and translational studies, offering insights into EGFR signaling dynamics and its role in cancer biology.
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