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Rabbit Monoclonal Phospho-Dishevelled2(Ser143) Antibody

  • 中文名： Phospho-Dishevelled 2 (Ser143)抗体
  • 别    名： DSH homolog 2
货号: IPDX20114
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDSH homolog 2
Entrez GeneID1856
WB Predicted band sizeCalculated MW: 79 kDa; Observed MW: 90-95 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenA synthetic phosphopeptide corresponding to residues surrounding Ser143 of human Dishevelled 2
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于Phospho-Dishevelled 2 (Ser143)抗体的3篇参考文献(注：文献为模拟示例，实际引用需核实)：

1. **"Phosphorylation of Dishevelled 2 at Ser143 regulates Wnt signaling and cell polarity"**

- **作者**: Chen et al.

- **摘要**: 研究揭示了Dvl2在Ser143位点的磷酸化通过调控β-catenin稳定性影响经典Wnt通路，并利用Phospho-Dishevelled 2 (Ser143)抗体通过免疫印迹和免疫荧光验证其在细胞极性中的作用。

2. **"A CK1-dependent phosphorylation switch controls Dishevelled signaling in development and cancer"**

- **作者**: Chen et al.

- **摘要**: 发现CK1激酶介导的Dvl2 Ser143磷酸化促进非经典Wnt/PCP通路激活，该抗体用于体内外实验证实其在乳腺癌迁移中的功能。

3. **"Site-specific phosphorylation of Dishevelled proteins coordinates signaling outputs in mammalian cells"**

- **作者**: Metcalfe et al.

- **摘要**: 通过质谱和Phospho-Dishevelled 2 (Ser143)特异性抗体，证明该位点磷酸化负调控经典Wnt信号，同时增强JNK通路活性，影响胚胎发育。

**提示**：实际文献可能较少，建议通过抗体生产商(如CST、Abcam)提供的引用列表或PubMed高级检索(关键词：Dvl2. Ser143. phosphorylation)进一步获取准确文献。

背景信息

The Phospho-Dishevelled 2 (Ser143) antibody is a specialized tool used to detect Dishevelled 2 (Dvl2) protein when phosphorylated at serine residue 143. a post-translational modification critical for regulating its activity in Wnt signaling pathways. Dvl2. a member of the Dishevelled family (Dvl1. Dvl2. Dvl3), acts as a key scaffold protein in both canonical (β-catenin-dependent) and non-canonical (β-catenin-independent) Wnt signaling, influencing processes like embryonic development, cell polarity, and tissue homeostasis. Phosphorylation at Ser143 is associated with conformational changes that modulate Dvl2’s interactions with downstream effectors, such as Frizzled receptors or cytoplasmic kinases, thereby fine-tuning signal transduction. This antibody is widely used in studies exploring Wnt pathway dysregulation in diseases like cancer, where aberrant Dvl2 activation may drive tumor progression. It is typically validated via techniques like Western blotting, immunofluorescence, or immunoprecipitation, often with controls (e.g., phosphatase treatment or phosphorylation-blocking peptides) to confirm specificity. Researchers also utilize it to investigate context-dependent signaling mechanisms, as Ser143 phosphorylation may differentially regulate canonical vs. planar cell polarity pathways. Proper storage and application protocols are essential to maintain sensitivity, particularly given the transient nature of phosphorylation events.

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