| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IGF1R; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD antigen CD221 |
| Entrez GeneID | 3480 |
| WB Predicted band size | Calculated MW: 155 kDa; Observed MW: 95 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Tyr1166 of human INSR/IGF-1R |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-IGF1 Receptor (Tyr1165/1166)抗体的3篇参考文献(注：文献中常用Tyr1165对应人IGF1R编号，与小鼠Tyr1166同源位点)：
1. **文献名称**: *"IGF-1 receptor phosphorylation in skeletal muscle cells following ligand binding"*
**作者**: S. Yamauchi et al.
**摘要**: 通过免疫沉淀和Western blot分析，验证了Phospho-IGF1R (Tyr1165/1166)抗体在检测IGF-1刺激下受体自磷酸化中的特异性，揭示其在胰岛素样生长因子信号传导中的关键作用。
2. **文献名称**: *"Targeting IGF-1 receptor signaling in triple-negative breast cancer"*
**作者**: M.L. Avivar-Valderas et al.
**摘要**: 使用该抗体证实三阴性乳腺癌细胞中IGF1R的持续激活(Tyr1165磷酸化)，并证明其与PI3K/AKT通路活化和耐药性相关，为靶向治疗提供依据。
3. **文献名称**: *"Phosphorylation of IGF-1 receptor tyrosine kinase associated with DNA damage response"*
**作者**: K. Nakamura et al.
**摘要**: 研究发现DNA损伤诱导IGF1R在Tyr1165位点的磷酸化，通过该抗体检测到磷酸化信号增强，提示IGF1R在细胞应激反应中的新功能。
(注：以上为模拟文献，实际研究中建议通过PubMed或Web of Science以关键词“Phospho-IGF1R Tyr1165”或“IGF1R Y1165 phosphorylation”检索最新论文。)
The phospho-IGF1 receptor (Tyr1166) antibody is a specialized tool used to detect the activated form of the insulin-like growth factor 1 receptor (IGF1R), a transmembrane tyrosine kinase receptor critical for cell growth, metabolism, and survival. IGF1R becomes activated upon binding to its ligands, IGF1 or IGF2. triggering autophosphorylation of specific tyrosine residues in its intracellular kinase domain. Tyr1166 (or Tyr1165 in some nomenclature systems) is a key phosphorylation site located in the activation loop of the receptor's β-subunit. This phosphorylation event stabilizes the active conformation of the kinase, enabling downstream signaling through pathways like PI3K/AKT and MAPK/ERK, which regulate cellular proliferation and apoptosis.
The phospho-specific antibody targets this phosphorylated Tyr1166 residue, allowing researchers to distinguish between the active and inactive states of IGF1R. It is widely used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study IGF1R activation in response to ligand stimulation, cellular stress, or oncogenic signaling. Dysregulation of IGF1R signaling is implicated in cancer progression, diabetes, and metabolic disorders, making this antibody valuable for both basic research and translational studies. By monitoring phosphorylation at Tyr1166. scientists can assess receptor activity in disease models, evaluate therapeutic interventions targeting IGF1R (e.g., kinase inhibitors), and explore mechanisms underlying drug resistance. Proper validation of the antibody's specificity is essential to avoid cross-reactivity with closely related receptors like the insulin receptor.
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