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产品描述：人过氧化物酶体增殖因子活化受体γ( PPAR-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PPARG含量。PPARG 即过氧化物酶体增殖物激活受体γ，是核激素受体家族成员。它在脂肪细胞分化、糖脂代谢、炎症反应等生理过程中发挥重要作用。研究机制多围绕其配体结合及下游信号通路展开，通过调节基因表达来影响细胞功能，是糖尿病等疾病药物研发的重要靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于科研领域中对PPAR - γ表达调控机制的研究，例如在肥胖、糖尿病或动脉粥样硬化等代谢性疾病模型中，评估药物干预、基因修饰或环境因素对PPAR - γ信号通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CIMT1 ELISA Kit; GLM1 ELISA Kit; NR1C3 ELISA Kit; Nuclear receptor subfamily 1 group C member 3 ELISA Kit; OTTHUMP00000185032 ELISA Kit; OTTHUMP00000185036 ELISA Kit; Peroxisome proliferator activated nuclear receptor gamma variant 1 ELISA Kit; Peroxisome proliferator activated receptor gamma 1 ELISA Kit; Peroxisome Proliferator Activated Receptor gamma ELISA Kit; Peroxisome proliferator-activated receptor gamma ELISA Kit; PPAR gamma ELISA Kit; PPAR-gamma ELISA Kit; PPARG ELISA Kit; PPARG_HUMAN ELISA Kit; PPARG1 ELISA Kit; PPARG2 ELISA Kit; PPARgamma ELISA Kit
缩写：PPARG
Uniprot No.：P37231
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PPAR-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 84-99 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 80-94 | |||||
回收率：
| The recovery of human PPAR-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-102 | ||||
| EDTA plasma (n=4) | 89 | 84-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.3152.2152.2652.11925001.8171.7171.7671.62112501.2571.1571.2071.0616250.7120.7020.7070.5613120.4180.4080.4130.2671560.3310.3210.3260.180780.2550.2450.2500.10400.1510.1410.146 | ||||||
货期：3-5 working days
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