首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠过氧化物酶体增殖物激活受体α(PPAR-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PPARA含量。PPARA，即过氧化物酶体增殖物激活受体α，是一种核受体，主要在肝脏、心肌、肾脏和骨骼肌等组织中表达。它参与调节能量代谢、氧化应激和炎症等多种生物活动，与动脉粥样硬化、冠心病、心肌梗死和心力衰竭等疾病密切相关。PPARA的研究机制涉及其与YAP-TEAD信号通路相互作用，促进肝增大和肝再生，以及通过调节血脂代谢和炎症反应来影响心血管疾病的发生发展。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外培养细胞、实验动物模型等科研场景中PPAR-α蛋白表达水平的动态监测，可支持脂代谢调控机制探索、药物干预效果评估等基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ppara ELISA Kit; Nr1c1 ELISA Kit; Ppar ELISA Kit; Peroxisome proliferator-activated receptor alpha ELISA Kit; PPAR-alpha ELISA Kit; Nuclear receptor subfamily 1 group C member 1 ELISA Kit
缩写：PPARA
Uniprot No.：P37230
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PPAR-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-100 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-101 | |||||
回收率：
| The recovery of rat PPAR-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 86-96 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.1832.1772.1802.068101.6541.6271.6411.52951.2401.2111.2261.1142.50.8590.8420.8510.7391.250.5460.5130.5300.4180.6250.3810.3730.3770.2650.3120.1660.1570.1620.05000.1110.1130.112 | ||||||
货期：3-5 working days
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