首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人骨膜蛋白/成骨细胞特异性因子2(POSTN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的POSTN含量。POSTN即原纤维蛋白，是一种细胞外基质蛋白。它参与胚胎发育、组织修复等生理过程。研究发现其在肿瘤等疾病中异常表达，可影响肿瘤的增殖、迁移和侵袭，还能调节肿瘤微环境，因此成为疾病治疗尤其是肿瘤治疗研究的潜在靶点。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于科研场景中探究POSTN在骨骼发育、组织纤维化或肿瘤微环境调控中的分子机制，也可用于疾病模型动物血清标志物分析或体外细胞培养上清中POSTN分泌水平的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fasciclin-I like ELISA Kit; MGC119510 ELISA Kit; MGC119511 ELISA Kit; OSF 2 ELISA Kit; OSF-2 ELISA Kit; OSF2 ELISA Kit; Osteoblast specific factor 2 (fasciclin I like) ELISA Kit; Osteoblast specific factor 2 ELISA Kit; Osteoblast specific factor ELISA Kit; Osteoblast-specific factor 2 ELISA Kit; PDLPOSTN ELISA Kit; Periodontal ligament specific periostin ELISA Kit; Periostin ELISA Kit; Periostin isoform thy2 ELISA Kit; Periostin isoform thy4 ELISA Kit; Periostin isoform thy6 ELISA Kit; Periostin isoform thy8 ELISA Kit; Periostin osteoblast specific factor ELISA Kit; PN ELISA Kit; POSTN ELISA Kit; POSTN_HUMAN ELISA Kit; RP11 412K4.1 ELISA Kit
缩写：POSTN
Uniprot No.：Q15063
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human POSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 90-95 | |||||
| 1:10 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human POSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 94 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.7882.8032.7962.6592002.1342.1542.1442.0071001.4621.5161.4891.352500.8750.8390.8570.720250.4370.4240.4310.29412.50.3540.3410.3480.2116.250.2450.2340.2400.10300.1380.1360.137 | ||||||
货期：3-5 working days
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