首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人对氧磷酶-3(PON3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PON3含量。PON3 是一个重要靶点。它属于对氧磷酶家族，广泛存在于高密度脂蛋白表面。研究发现 PON3 具有抗氧化、抗炎等特性，其机制可能与催化水解脂质过氧化物、调节细胞信号通路等有关，在心血管疾病等领域有潜在研究价值。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于基础科研领域，可助力探索PON3在心血管疾病、糖尿病、非酒精性脂肪肝等代谢异常相关疾病中的分子机制，或用于评估药物干预对脂质过氧化及炎症反应的影响，为体外实验研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2810004E20 ELISA Kit; AI786302 ELISA Kit; MGC95026 ELISA Kit; Paraoxonase 3 ELISA Kit; Pon3 ELISA Kit; PON3_HUMAN ELISA Kit; Serum paraoxonase/lactonase 3 ELISA Kit
缩写：PON3
Uniprot No.：Q15166
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PON3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 96 | ||||
| Range % | 90-106 | |||||
| 1:2000 | Average % | 91 | ||||
| Range % | 82-99 | |||||
| 1:4000 | Average % | 89 | ||||
| Range % | 80-97 | |||||
| 1:8000 | Average % | 93 | ||||
| Range % | 85-102 | |||||
回收率：
| The recovery of human PON3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 85-105 | ||||
| EDTA plasma (n=4) | 95 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4722.4112.4422.35651.8731.8601.8671.7812.51.2641.2431.2541.1681.250.7210.7140.7180.6320.6250.5420.5680.5550.4690.3120.2820.2960.2890.2030.1560.2060.2170.2120.12600.0850.0860.086 | ||||||
货期：3-5 working days
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