首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠对氧磷酶-1(PON1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PON1含量。PON1（对氧磷酶1）是一种与高密度脂蛋白（HDL）结合的水解酶，具有抗氧化和代谢有机磷农药的功能。其研究机制涉及PON1在有机磷农药代谢中的作用，以及与心血管疾病和动脉硬化的关系。PON1的基因多态性影响其活性，进而影响个体对有机磷农药毒性和心血管疾病的风险。试剂盒检测范围为15.6 μU/mL-1000 μU/mL，适用于多种科研场景下PON1水平的精准分析，适用于毒理学研究、氧化应激模型评估及代谢相关疾病的分子机制探索，为心血管、肝脏功能及环境毒物暴露等领域的科学研究提供高效工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pon1 ELISA Kit; Pon ELISA Kit; Serum paraoxonase/arylesterase 1 ELISA Kit; PON 1 ELISA Kit; EC 3.1.1.2 ELISA Kit; EC 3.1.1.81 ELISA Kit; EC 3.1.8.1 ELISA Kit; Aromatic esterase 1 ELISA Kit; A-esterase 1 ELISA Kit; Serum aryldialkylphosphatase 1 ELISA Kit
缩写：PON1
Uniprot No.：P55159
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 μU/mL-1000 μU/mL
灵敏度：3.9 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:800 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:1600 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of rat PON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μU/mlOD1OD2AverageCorrected10002.7332.7992.7662.6165002.2742.2202.2472.0972501.7181.7081.7131.5631251.1031.1231.1130.96362.50.6410.6820.6620.51231.20.3510.3620.3570.20715.60.2450.2360.2410.09100.1500.1490.150 | ||||||
货期：3-5 working days
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