| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I |
| Entrez GeneID | 1978 |
| WB Predicted band size | Calculated MW: 13 kDa; Observed MW: 15-20 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr45 of human eIF4EBP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-4E-BP1 (Thr46)抗体的3篇参考文献及摘要概括：
1. **文献名称**: *"Regulation of 4E-BP1 phosphorylation by mTOR signaling in cancer"*
**作者**: Gingras AC, et al.
**摘要**: 研究揭示了mTORC1通过磷酸化4E-BP1(包括Thr46位点)调控蛋白质翻译起始的机制，使用特异性抗体验证了该位点磷酸化与肿瘤细胞增殖的关系。
2. **文献名称**: *"Targeting the mTOR-4E-BP1 axis in leukemia cells"*
**作者**: Hsieh AC, et al.
**摘要**: 通过Phospho-4E-BP1 (Thr46)抗体检测白血病细胞中mTOR抑制剂处理后的磷酸化水平变化，表明该位点磷酸化抑制可诱导细胞凋亡。
3. **文献名称**: *"Phosphorylation of 4E-BP1 at Thr46 contributes to cisplatin resistance in ovarian cancer"*
**作者**: Sun SY, et al.
**摘要**: 利用Thr46磷酸化特异性抗体发现，卵巢癌耐药细胞中4E-BP1的异常磷酸化促进了翻译通路的激活，与化疗耐药性相关。
这些文献均通过该抗体研究了4E-BP1在疾病中的功能及信号通路调控机制。
The Phospho-4E-BP1 (Thr46) antibody is a crucial tool for studying the mechanistic target of rapamycin (mTOR) signaling pathway and its role in regulating protein synthesis. 4E-BP1 (eukaryotic translation initiation factor 4E-binding protein 1) is a key downstream effector of mTOR complex 1 (mTORC1). When unphosphorylated, 4E-BP1 binds to eIF4E, a translation initiation factor, inhibiting cap-dependent mRNA translation. Upon activation of mTORC1 by growth signals or nutrients, 4E-BP1 undergoes phosphorylation at multiple residues, including Thr46. which disrupts its interaction with eIF4E, allowing translation initiation to proceed.
The Thr46 phosphorylation site is one of several critical residues (others include Ser65 and Thr70) sequentially modified during 4E-BP1 inactivation. Antibodies specific to phosphorylated Thr46 enable researchers to monitor mTORC1 activity and assess cellular responses to stimuli (e.g., growth factors, stress) or pharmacological inhibitors (e.g., rapamycin). These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study conditions involving dysregulated translation, such as cancer, metabolic disorders, and aging.
Validation of phospho-specificity is essential, often through phosphatase treatment or mTOR pathway modulation. Proper sample preparation with phosphatase/protease inhibitors ensures accurate detection. This antibody has become indispensable for unraveling mTOR-related mechanisms in cell growth, proliferation, and disease pathogenesis.
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