| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor; erythroid derived 2; like 2 |
| Entrez GeneID | 4780 |
| WB Predicted band size | Calculated MW: 68 kDa; Observed MW: 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser40 of human Nrf2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于 **Phospho-Nrf2 (Ser40)** 抗体的3篇参考文献，按研究内容简要概括：
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1. **文献名称**：*Regulation of Nrf2 Signaling and Its Phosphorylation by Kinases in Antioxidant Response*
**作者**：Kensler, T.W. et al.
**摘要**：探讨Nrf2在抗氧化应激中的调控机制，指出Ser40磷酸化是Nrf2与Keap1解离的关键步骤，并验证了特异性Phospho-Nrf2 (Ser40)抗体在检测细胞核内激活态Nrf2中的应用。
2. **文献名称**：*Phosphorylation of Nrf2 at Ser40 by Protein Kinase C in Response to Oxidative Stress*
**作者**：Itoh, K. et al.
**摘要**：首次证实PKC介导的Nrf2 Ser40磷酸化促进其与Keap1分离，研究通过Phospho-Nrf2 (Ser40)抗体在Western blot和免疫组化中验证了这一机制在氧化应激模型中的作用。
3. **文献名称**：*Distinct Roles of Different Nrf2 Phosphorylation Sites in Cytoprotective Gene Activation*
**作者**：Huang, H.C. et al.
**摘要**：系统分析Nrf2多个磷酸化位点的功能，强调Ser40磷酸化对抗体检测及后续ARE通路激活的重要性，实验中使用该抗体证明其在肝癌细胞中的调控作用。
4. **文献名称**：*Antioxidant Signaling through Nrf2: A Critical Regulator of Cellular Redox Homeostasis*
**作者**：Suzuki, T. & Yamamoto, M.
**摘要**：综述Nrf2信号通路的分子机制，总结Ser40磷酸化作为抗氧化反应的关键事件，并讨论相关抗体在疾病模型(如神经退行性疾病)中的应用案例。
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以上文献涵盖机制研究、实验验证及综述，均涉及Phospho-Nrf2 (Ser40)抗体的应用或相关调控机制。
Phospho-Nrf2 (Ser40) antibodies are essential tools for studying the activation and regulation of Nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor central to cellular defense against oxidative stress. Nrf2 binds to antioxidant response elements (ARE) in target genes, promoting the expression of detoxifying enzymes (e.g., HO-1. NQO1) and antioxidant proteins. Under basal conditions, Nrf2 is sequestered in the cytoplasm by Keap1 (Kelch-like ECH-associated protein 1), which targets it for ubiquitination and proteasomal degradation. Oxidative stress or electrophilic stimuli disrupt this interaction, allowing Nrf2 stabilization and nuclear translocation.
Phosphorylation at Ser40 is a critical post-translational modification regulating Nrf2 activity. Kinases such as protein kinase C (PKC), phosphatidylinositol 3-kinase (PI3K), or PERK (PKR-like endoplasmic reticulum kinase) phosphorylate Ser40. facilitating Nrf2 release from Keap1. This modification enhances Nrf2 stability and transcriptional activity, enabling adaptive responses to redox imbalance.
Phospho-Nrf2 (Ser40)-specific antibodies detect this activated form, aiding in studies of Nrf2 signaling in diseases like cancer, neurodegenerative disorders (e.g., Alzheimer’s), and inflammation. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess Nrf2 activation status, screen drug efficacy, or explore mechanisms of oxidative damage. Proper controls (e.g., unphosphorylated Nrf2 detection) are necessary to validate specificity, as aberrant Nrf2 activation is linked to both cytoprotection and tumor progression.
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