| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rb2; P130 |
| Entrez GeneID | 5934 |
| WB Predicted band size | Calculated MW: 128 kDa; Observed MW: 128 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr986 of human p130 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-Rb2 p130 (Thr986)抗体的3篇参考文献示例(注：文献为假设性示例，实际需根据具体数据库检索验证)：
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1. **文献名称**: "Phosphorylation of p130 at Threonine 986 regulates cell cycle exit and differentiation"
**作者**: Smith EJ, et al.
**摘要**: 研究Thr986位点的磷酸化在细胞周期退出和分化中的作用，利用Phospho-Rb2 p130 (Thr986)抗体通过Western blot和免疫荧光技术，发现该位点的磷酸化与G0期细胞周期停滞相关。
2. **文献名称**: "Development of a phospho-specific antibody targeting Rb2/p130 Thr986 and its application in cancer prognosis"
**作者**: Zhang Y, et al.
**摘要**: 描述该抗体的制备与验证，包括抗原设计、特异性检测(ELISA、免疫沉淀)及在乳腺癌组织中的临床应用，表明高磷酸化水平与不良预后相关。
3. **文献名称**: "Kinase-dependent regulation of Rb2/p130 Thr986 phosphorylation in lung cancer cells"
**作者**: Lee H, et al.
**摘要**: 探讨CDK4/6激酶对Thr986磷酸化的调控，使用该抗体分析肺癌细胞系中磷酸化动态变化，揭示其与肿瘤增殖和药物敏感性的关联。
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**建议**：若需真实文献，可通过PubMed或Google Scholar搜索关键词“Phospho-Rb2 p130 Thr986 antibody”，并筛选应用或开发该抗体的研究。部分抗体供应商(如Cell Signaling Technology)的产品手册也可能提供相关参考文献。
Phospho-Rb2 p130 (Thr986) antibody is designed to detect the retinoblastoma-like protein 2 (p130. also known as RBL2) when phosphorylated at threonine 986. Rb2/p130 is a member of the retinoblastoma (Rb) tumor suppressor family, which includes Rb1/p105 and p107. These proteins regulate cell cycle progression by interacting with E2F transcription factors, inhibiting their activity and blocking G1-to-S phase transition. Phosphorylation of Rb2/p130 at specific residues, including Thr986. modulates its function by disrupting its binding to E2F, thereby promoting cell cycle progression. This phosphorylation event is primarily mediated by cyclin-dependent kinases (CDKs) during the G1 phase or in response to mitogenic signals.
The Thr986 phosphorylation site is associated with cell cycle-dependent regulation, where hypophosphorylated Rb2/p130 acts as a growth suppressor in quiescent or differentiated cells, while hyperphosphorylation inactivates its tumor-suppressive role. Aberrant phosphorylation of Rb2/p130 has been linked to cancer progression, as loss of proper cell cycle control can lead to uncontrolled proliferation. Researchers use Phospho-Rb2 p130 (Thr986) antibodies in techniques like Western blotting, immunofluorescence, or immunohistochemistry to study cell cycle dynamics, cancer mechanisms, or therapeutic responses. Specificity validation (e.g., via knockout controls or phosphatase treatment) is critical to ensure accurate detection, as cross-reactivity with other Rb family members or phosphorylation sites may occur. This antibody serves as a tool to explore Rb2/p130's role in tumor suppression, differentiation, and CDK inhibitor therapies.
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