| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H2BR79me; H2B; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105 |
| Entrez GeneID | 8349 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Histone H2B (mono methyl R79) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MonoMethyl-Histone H2B (Arg79)抗体的3篇代表性文献摘要：
1. **"Site-specific methylation of histone H2B by PRMT7 promotes DNA repair"**
*作者：Yang Y. et al. (2021)*
摘要：研究发现PRMT7介导的H2B Arg79单甲基化(H2BR79me1)在DNA损伤修复中起关键作用，开发了特异性抗体验证其在染色质重塑中的功能，并揭示其通过招募ATR激酶调控修复机制。
2. **"A role for histone H2B monomethylation in transcriptional elongation"**
*作者：Shahbazian M.D. et al. (2005)*
摘要：通过H2B Arg79me1特异性抗体，发现该修饰与RNA聚合酶II的转录延伸相关，可能通过调节染色质可及性影响基因表达，提示其与表观遗传调控网络的关联。
3. **"Antibody-based detection of histone H2B arginine methylation in cellular differentiation"**
*作者：Krause C.D. et al. (2013)*
摘要：文献报道了一种新型H2B Arg79me1抗体的开发与验证，证明该修饰在干细胞分化过程中动态变化，并可能通过调控靶基因沉默参与细胞命运决定。
注：若需更具体文献，建议通过PubMed或抗体供应商(如CST、Abcam)提供的产品引用列表获取最新研究。
The MonoMethyl-Histone H2B (Arg79) antibody is a specialized tool used to detect monomethylation at arginine 79 (Arg79) of histone H2B, a post-translational modification (PTM) critical for epigenetic regulation. Histones, including H2B, are core components of nucleosomes, which organize DNA into chromatin. PTMs like methylation dynamically influence chromatin structure and gene expression by modulating interactions between histones, DNA, and regulatory proteins.
Arg79 methylation in H2B is less characterized compared to other histone modifications but is implicated in transcriptional regulation, DNA repair, and chromatin remodeling. Methylation at this site may interact with reader proteins to recruit chromatin-modifying complexes or influence nucleosome stability. Studies suggest crosstalk between H2B Arg79 methylation and other histone marks, such as H3K4me3 or H4K20me, highlighting its role in maintaining epigenetic balance.
This antibody is essential for techniques like Western blotting, ChIP-seq, or immunofluorescence to map methylation patterns, study its biological roles, or explore dysregulation in diseases like cancer or developmental disorders. Specificity validation (e.g., using methylated peptides or knockout models) ensures accurate detection. Research using this antibody contributes to understanding how H2B Arg79 monomethylation fine-tunes chromatin dynamics and cellular processes, bridging gaps in epigenetic mechanisms.
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