| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a |
| Entrez GeneID | 8350 |
| clone | 5H6 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of Histone H3 (Di Methyl Lys27) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于H3K27me2(Dimethyl-Histone H3 Lys27)抗体的经典文献摘要，供参考：
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1. **文献名称**: *Histone H3K27 trimethylation and H3K4 methylation define different chromatin states in Polycomb silencing*
**作者**: Boyer LA et al.
**摘要**: 该研究通过ChIP-seq技术，使用H3K27me2/me3抗体揭示Polycomb复合物(PRC)介导的基因抑制机制，发现H3K27二甲基化与三甲基化在胚胎干细胞分化中具有不同的表观遗传调控功能。
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2. **文献名称**: *Role of histone H3 lysine 27 methylation in Polycomb-group silencing*
**作者**: Cao R et al.
**摘要**: 本文利用H3K27me2特异性抗体进行染色质免疫沉淀实验，证明EZH2(PRC2核心催化亚基)介导的H3K27二甲基化和三甲基化是维持干细胞多能性和基因沉默的关键表观遗传标记。
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3. **文献名称**: *A histone H3 lysine 27-specific antibody validates Drosophila Polycomb function*
**作者**: Pengelly AR et al.
**摘要**: 作者开发并验证了H3K27me2抗体的特异性，通过果蝇模型证明H3K27二甲基化在发育基因调控中与Polycomb抑制复合物协同作用，且其分布模式与三甲基化存在差异。
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4. **文献名称**: *Histone modifications: from genome-wide maps to functional insights*
**作者**: Bernstein BE et al.
**摘要**: 该综述系统总结了H3K27me2等组蛋白修饰的检测方法(如抗体特异性验证)，并探讨其在染色质结构动态调控及癌症等疾病中的潜在作用机制。
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提示：具体实验引用时建议通过PubMed或Google Scholar核对最新文献，关注抗体验证方法(如Western blot/ChIP的示例数据)以确保可靠性。
The DiMethyl-Histone H3 (Lys27) antibody specifically detects histone H3 proteins dimethylated at lysine 27 (H3K27me2), a post-translational modification critical for epigenetic regulation. Histone H3 is a core component of nucleosomes, and lysine methylation modulates chromatin structure and gene expression. H3K27me2 is associated with transcriptional repression and serves as a dynamic marker of chromatin states. Unlike the well-characterized H3K27me3 (trimethylation), which is strongly linked to Polycomb Repressive Complex 2 (PRC2)-mediated gene silencing in development and cancer, H3K27me2 is more broadly distributed and may play roles in maintaining facultative heterochromatin or priming regions for PRC2 activity.
This antibody is widely used in chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting to study epigenetic mechanisms in cellular differentiation, oncogenesis, and stem cell biology. Aberrant H3K27 methylation is implicated in diseases, including malignancies like gliomas and lymphomas, where altered PRC2 activity disrupts normal methylation patterns. Researchers rely on the specificity of H3K27me2 antibodies to distinguish between methylation states, often validating results with demethylase treatments or knockouts. Cross-reactivity with H3K27me1/me3 must be ruled out to ensure accurate interpretation. Understanding H3K27me2 dynamics provides insights into how cells balance gene activation and repression during development and disease progression.
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